R26-CAG-Cas9

Nomenclature

C57BL/6Smoc-Gt(ROSA)26Sortm1(CAG-Cas9)Smoc

Cat. NO.

NM-KI-00120

Strain State

Repository Live

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Gene Summary

Gene Symbol
Gt(ROSA)26Sor

Synonyms

R26，ROSA26，AV258896，Gtrgeo26，Gtrosa26，Thumpd3as1

NCBI ID

14910

MGI ID

104735

Ensembl ID

ENSMUSG00000086429

Pubmed

Gt(ROSA)26Sor

Model Description

These mice were established by crossing R26-(CAG-LSL-Cas9) and EIIA mice together.
Research Application：gene editing, gene modification

Validation Data


image.png

Fig1. Analysis of Cas9 expression profile by Western Blot. The CAG-Cas9 KI mice express Cas9 in heart, liver, spleen, lung, kidney, stomach, cortex, cerebellum, testis and muscle.

J***: Cas9 KI mice from other peers 

CAG-Cas9: R26-CAG-Cas9 mice (NM-KI-00120) obtained by R26-CAG-LNL-Cas9(NM-KI-00038)  crossed with Ella-Cre

Cas9-krab: R26-CAG-3XKRAB-dCas9-IRES-EGFP（NM-KI-18007）

image.png

Fig2. Detection of CAS9 expression in R26-CAG-CAS9 mice by WB. 

Abbr. M, marker; WT, wild type; HO, homozygous; WAT, white adipose tissue; BAT, brown adipose tissue. 

Note. 60 μg of total protein were loaded for BAT and skeletal muscle, and 20 μg for other tissues.

image.png

Fig.3 Detection of CAS9 expression in T cells in R26-CAG-CAS9 mice by WB. 

Abbr. M, marker; WT, wild type; HO, homozygous.

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