Introduction
Graft-versus-host disease (GvHD) is a major complication of the widespread use of hematopoietic stem cell transplantation, caused by the immune attack of donor T cells within the graft against the recipient's tissues. Mouse models of GvHD can be used to simulate the clinical symptoms of GvHD following allogeneic bone marrow transplantation, as well as to study T cell regulation, tolerance induction, and autoimmune diseases.
Disease models
Transplantation of appropriate donor bone marrow and lymphocytes into sublethally irradiated M-NSG mice can induce various symptoms of GvHD, including multisite inflammation, significant weight loss, and mouse death.
Fig.1 Validation of the Acute GvHD Model.
Female M-NSG mice (n= 6, 6-8 weeks old) were irradiated with 1 Gy and then inoculated with 1×10^7 huPBMC cells to induce acute GvHD. After treatment with Abatacept, the survival rate of the mice significantly increased.
