Atopic Dermatitis Model

Introduction

Atopic dermatitis (AD) is a chronic, relapsing inflammatory skin disorder characterized by persistent pruritus. It typically occurs in children and adolescents but may also manifest in adults. The pathogenesis of atopic dermatitis is complex and may involve multiple factors, including genetics, environment, and immune dysregulation.

Disease models

The SMOC has long been dedicated to autoimmune disease research and has developed a variety of mouse models of atopic dermatitis using agents such as oxazolone (OXA) and 2,4-dinitrofluorobenzene (DNFB), providing powerful tools for the efficacy evaluation and safety assessment of related drugs.

OXA induced AD model in hIL4/hIL4R mice

image.png

Fig.1 OXA induced AD model in hIL4/hIL4R mice. (A) body weight (B) body weight change.

image.png

Fig.2 OXA induced AD model in hIL4/hIL4R mice. (A) gross observation on Day 21  (B) ear thickness (C) clinical score of skin.

image.png

Fig.3 OXA induced AD model in hIL4/hIL4R mice. (A) serum IgE (B) spleen weight.

image.png

Fig.4 OXA induced AD model in hIL4/hIL4R mice. (A) Pathology photos (B) Pathology score.

DNFB induced AD model in hIL4/hIL4R mice

image.png

Fig.1 Body weight of hIL4/hIL4Ra mice treated with dupilumab. *P<0.05.

image.png

Fig.2 Dupilumab ameliorate overall atopic dermatitis activity in DNFB-induced AD Model. *P<0.05, **P<0.01, ***P<0.001.

image.png

Fig.3 Dupilumab treatment significantly reduced IgE levels in serum and scratching times. (A) day10 serum IgE (B) day16 serum IgE (C) scratch times on Day 12 (D) scratch times on Day 14. *P<0.05, **P<0.01, ***P<0.001.

image.png

Fig.4 Dupilumab significantly mitigates inflammatory cell infiltration in lesioned skin on day 14. (A) dorsal image on day14; (B) Representative pathology images; (C) Inflammatory cell infiltration score; (D) neutrophils score; (E) eosinophils score; (F) epidermis thickness; (G) dermis thickness. *P<0.05, **P<0.01, ***P<0.001.

DNFB induced AD model in BALB/c mice

image.png

image.pngD

image.png


Fig.1 DNFB induced AD model in BALB/c mice. (A) body weight (B) ear thickness (C) clinical score of skin (D) photographs of the mouse’s back and H&E-stained histological sections of the ear skin (E) serum IgE (E–H)statistical analysis of the degree of immune cell infiltration and dermal thickness.

OXA induced AD model in C57BL/6 mice

image.png

Fig.1 OXA induced AD model in C57BL/6 mice. (A–B) Changes in ear thickness and clinical scores in mice. (C) Photographs of the mice’s backs. (D–E) Measurement of serum IgE levels and spleen weight in mice. (F–H) H&E-stained histological sections of mouse skin and statistical analysis of the degree of immune cell infiltration and dermal thickness. The results indicate that dexamethasone significantly alleviated the skin lesions associated with OXA-induced specific dermatitis.


MC903-induced AD model in C57BL/6 mice

image.png

Fig1. The efficacy of Crisaborole on MC903-induced AD model in C57BL/6 wild-type mice. (A) body weight (B) body weight change 

image.png

Fig2. The efficacy of Crisaborole on MC903-induced AD model in C57BL/6 wild-type mice. (A) ear thickness (B) ear thickness change

image.png

Fig3. The efficacy of Crisaborole on MC903-induced AD model in C57BL/6 wild-type mice. (A) erythema score (B) skin thickness score (C) scabby score (D) clinical score

image.png

Fig4. The efficacy of Crisaborole on MC903-induced AD model in C57BL/6 wild-type mice. (A) serum mIgE (B) spleen index

image.png

Fig5. The efficacy of Crisaborole on MC903-induced AD model in C57BL/6 wild-type mice. (A) ear mTGF-β (B) ear mIL13 (C) ear mIL5 (D) ear mIL25 (E) ear mIL2 (F) ear mIL33 (G) ear mTSLP (H) ear mTNFa (I) ear mIL8

image.png

Fig6. The efficacy of Crisaborole on MC903-induced AD model in C57BL/6 wild-type mice. (A) inflammatory cell infiltration score (B) neutrophils score (C) eosinophils score (D) dermis thickness (E) epidermis thickness (F) typical pathology image

南模生物Email Back to top 点击跳转到顶部