SmocMab Fully-human Antibody Transgenic Mouse

Key features of SmocMab platform:

  • Rapid Antibody Discovery: Tailored antibodies specific to your antigen in just 3 months. 

  • Dual-Technology: Combines fully humanized mice with knockout (KO) or point mutation (PM) homozygous models. 

  • Proven Success: Greatly shortens the fully antibody generation timeline. 

  • Broad Applications: Ideal for oncology, auto-immune diseases, and more.


You can order SmocMab mice for your research, we also provide CRO service based on SmocMab Mice, such as pharmaceutical research and development services, research and development of antibodies, early evaluation in the field of implementation of new drugs, development of pharmaceutical preparations and pharmaceuticals, biological research and analysis, pharmaceutical evaluation, etc.


 Schematic of Humanization in The SmocMab Mouse

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Fig1. Mouse variable regions of the heavy and k light chains are replaced by the human counterparts in situ.


Intact Immune System and Normal B-cell Development

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Fig2. Analysis of leukocytes cell subpopulation in Spleen.


Highly Similar Immune Responses Compared to WT Mouse

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Fig3. Detection of antibody titer in serum after three antigen immunizations in Smocmab(A) and C57BL/6(B).


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Fig4. Detection of antibody titer in serum after 1st or 2nd boost  in Smocmab(A) and C57BL/6(B). 


Efficient somatic hypermutation

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Fig5. SmocMab Heavy Chain CDR3 Amino Acid Usage Frequency.

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Fig6. SmocMab Kappa Chain CDR3 Amino Acid Usage Frequency.

High percentage of positive clones

  • Hybridoma technology

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    Fig7. Detection of antibody titer.After fusion, each project was plated on 40 plates. ELISA screening was performed. 169 and 161 supernatants were respectively selected for expansion.

  • Single B-cell cloning technique

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    Fig.8 Detection of cell culture supernate titer. 

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    Fig9. By further purifying the culture supernatant, antibodies with a purity exceeding 97% were obtained and subjected to titre testing.

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    Fig10. Antibodies obtained from SmocMab mice via single B-cell cloning technology exhibit high affinity.

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    Fig11. Antibodies obtained from SmocMab mice via single B-cell cloning technology exhibit high sequence diversity.

High binding to target protein

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Fig12. Binding affinity analysis of ligand to antalyte using Surface Plasmon Resonance (SPR). 


Higher cell-binding activity

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Fig13. Affinity evaluation of tested antibodies on target antigen highly expressed cell lines. 


Capable of targeting and penetrating BBB

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Fig14. In vivo evaluation of antibodies in mice. 


Exhibits high affinity for cynomolgus monkey target

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Fig14. Lead molecule exhibits higher affinity binding to immobilized cynomolgus protein.

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