hIGHA1(2)

Fig.1 Detection of IGHA1 expression in lymph node and colon by RT-PCR. Tissue RNA was extracted from 7-week-old wild-type C57BL/6 mice (WT, 2 males and 2 females) and homozygous hIGHA1(2) knockin mice (HO, 2 males and 2 females), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mRNA primers. Mouse Igha mRNA (135 bp) was detectable only in wild-type C57BL/6 mice. Human IGHA1 mRNA (211 bp) was detectable only in homozygous hIGHA1(2) knockin mice but not in wild-type mice.

Fig.2 Detection of IgA expression in serum by ELISA. Serum was collected from wild-type C57BL/6 mice (WT) and homozygous hIGHA1(2) knockin mice (HO) (n=2, 7 weeks old). Protein expression level of IgA was analyzed by ELISA. Human IgA was exclusively detectable in homozygous hIGHA1(2) knock-in mice. N.D, not detected.