Cd8a-IRES-Luc-2A-EGFP

Nomenclature

C57BL/6Smoc-Cd8aem1(V5-IRES-luci-2A-EGFP)Smoc

Cat. NO.

NM-KI-18030

Strain State

Embryo cryopreservation

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Gene Summary

Gene Symbol
Cd8a

Synonyms

Ly-2; Ly-B; Ly-35; Lyt-2; BB154331

NCBI ID

12525

MGI ID

88346

Ensembl ID

ENSMUSG00000053977

Pubmed

Cd8a

Human Ortholog

CD8A

Model Description

A V5-IRES-luci-2A-EGFP expression cassette was inserted before the stop codon TAA of mouse Cd8a gene to establish a multi-labeled knockin model with V5, luciferase and EGFP fluorescent protein.
Research Application：gene tracing

Validation Data

Cd8a-V5-IRES-Akaluc-2A-EGFP mouse model_05.png

Figure 1  In the absence of any stimulation, the in vivo imaging system detected that Cd8aV5-IRES-luci-2A-EGFP/+ mice showed significant fluorescence.

Cd8a-V5-IRES-Akaluc-2A-EGFP mouse model_06.png

Figure 2  In vivo imaging validation of MC38 tumors in Cd8aV5-IRES-luci-2A-EGFP/+ reporter mice treated or not with a PD-1 mAb. After 1 day of stimulation with PD1 antibody, the fluorescence was significantly enhanced.

Cd8aV5-IRES-luci-2A-EGFP-3.png

Figure 3  EGFP expression analysis in  PBMC of Cd8aV5-IRES-luci-2A-EGFP/+ mice by flow cytometry.

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