hIL2RA/hIL2RB/hIL2RG

Fig1. Detection of IL2RA expression in thymus and spleen by RT-PCR. 

Wild type: only one band at 350 bp with primers F1/R1(mIl2ra);

Homozygous: only one band at 318 bp with primers F2/R2(hIL2RA); 

Abbr.. M, DNA marker; HO, homozygous; HE, heterozygous; WT, wild type.

Fig2. Detection of IL2RB expression in thymus and spleen by RT-PCR. 

Wild type: only one band at 360 bp with primers F1/R1(mIl2rb);

Homozygous: only one band at 281 bp with primers F2/R2(hIL2RB); 

Abbr.. M, DNA marker; HO, homozygous; HE, heterozygous; WT, wild type.

Fig3. Detection of IL2RG expression in thymus and spleen by RT-PCR. 

Wild type: only one band at 400 bp with primers F1/R1(mIl2rg);

Homozygous: only one band at 263 bp with primers F2/R2(hIL2RG); 

Abbr.. M, DNA marker; HO, homozygous; HE, heterozygous; WT, wild type.

Fig.4 Human IL-2 induced the phosphorylation of Stat5 in CD4+ T cells, CD8+ T cells, NK cells, and Treg in the C57BL/6 WT mouse (n=2, 6 weeks old), hIL2RA/hIL2RB/hIL2RG knockin mice (n=2, 14 weeks old) and hIL2/hIL2RA/hIL2RB/hIL2RG knockin mice (n=2, 17 weeks old). 

Splenocytes were collected from the spleen, treated with different concentrations of human IL-2 for 15 min and analyzed by flow cytometry. As shown in the figure, human IL-2 induced pSTAT5 in CD4+ T cells, CD8+ T cells, NK cells, and Treg.

Fig.5 Mouse IL-2 induced the phosphorylation of Stat5 in CD4+ T cells, CD8+ T cells, NK cells, and Treg in the C57BL/6 WT mouse (n=2, 6 weeks old), hIL2RA/hIL2RB/hIL2RG knockin mice (n=2, 14 weeks old) and hIL2/hIL2RA/hIL2RB/hIL2RG knockin mice (n=2, 17 weeks old). 

Splenocytes were collected from the spleen, treated with different concentrations of mouse IL-2 for 15 min and analyzed by flow cytometry. As shown in the figure, mouse IL-2 induced pSTAT5 in CD4+ T cells, CD8+ T cells, NK cells, and Treg. However, the activation of mouse IL-2 is weaker than that of human IL-2 in hIL2RA/hIL2RB/hIL2RG knockin mice and hIL2/hIL2RA/hIL2RB/hIL2RG knockin mice.

Fig.6 Human IL-15 induced the phosphorylation of Stat5 in CD4+ T cells, CD8+ T cells, NK cells and Treg in the C57BL/6 WT mouse (n=2, 6 weeks old), hIL2RA/hIL2RB/hIL2RG knockin mice (n=2, 14 weeks old) and hIL2/hIL2RA/hIL2RB/hIL2RG knockin mice (n=2, 17 weeks old). 

Splenocytes were collected from the spleen, treated with different concentrations of human IL-15 for 15 min, and analyzed by flow cytometry. As shown in the figure, human IL-15 induced pSTAT5 in CD4+ T cells, CD8+ T cells, NK cells, and Treg.

Fig.7 Mouse IL-15 induced the phosphorylation of Stat5 in CD4+ T cells, CD8+ T cells, NK cells and Treg in the C57BL/6 WT mouse (n=2, 6 weeks old), hIL2RA/hIL2RB/hIL2RG knockin mice (n=2, 14 weeks old) and hIL2/hIL2RA/hIL2RB/hIL2RG knockin mice (n=2, 17 weeks old). 

Splenocytes were collected from the spleen, treated with different concentrations of human IL-15 for 15 min, and analyzed by flow cytometry. As shown in the figure, human IL-15 induced pSTAT5 in CD4+ T cells, CD8+ T cells, NK cells, and Treg.

Fig.8 Analysis of the phosphorylation of Stat5 in CD4+ T cells, CD8+ T cells, and Treg in human PBMC. 

PBMC were treated with different concentrations of human IL-2, human IL-15, mouse IL-2, and mouse IL-15 for 15 min, and analyzed by flow cytometry. As shown in the figure, human IL-2, human IL-15, and mouse IL-2 induced pSTAT5 in CD4+ T cells, CD8+ T cells, and Treg in a dose dependent manner. However, the activation of mouse IL-2 is weaker than that of human IL-2 and human IL-15.