Lgr5-EGFP-IRES-CreERT2

Fig. 1 CreERT2-mediated recombination in the stomach of Lgr5^{CreERT2 /+}; Rosa26^tdTomato/+ mouse. TdTomato(red) expression can be detected in the gastric mucosa and gastric crypt of Lgr5^{CreERT2 /+}; Rosa26^tdTomato/+ mouse. EGFP(green) expression can not be observed.

Fig. 2 CreERT2-mediated recombination in the small intestine of Lgr5^{CreERT2 /+}; Rosa26^tdTomato/+ mouse. TdTomato(red) expression can be detected in the mucous membrane epithelial cells and crypt cells of small intestine derived from Lgr5^{CreERT2 /+}; Rosa26^tdTomato/+ mouse. EGFP(green) expression can not be observed.

Fig. 3 CreERT2-mediated recombination in the large intestine of Lgr5^{CreERT2 /+}; Rosa26^tdTomato/+ mouse. TdTomato(red) expression can be detected in the mucous membrane epithelial cells and crypt cells of large intestine derived from Lgr5^{CreERT2 /+}; Rosa26^tdTomato/+ mouse. EGFP(green) expression can not be observed.

Fig. 4 CreERT2-mediated recombination in the hair follicles of Lgr5^{CreERT2 /+}; Rosa26^tdTomato/+ mouse. TdTomato(red) expression can be detected in the hair follicles and individual cells of subcutaneous tissue derived from Lgr5^{CreERT2 /+}; Rosa26^tdTomato/+ mouse. EGFP(green) expression can not be observed.

Fig. 5 Detection of tdTomato(red) and GFP(green) in various tissues of Lgr5^CreERT2/+; Rosa26^tdTomato/+ mice. CreERT2 mediated recombination can be detected in the glandular stomach, large intestine, small intestine and hair follicles. Some leakiness were detected prior to tamoxifen exposure. TdTomato expression can not be observed in the brain, pituitary gland, thymus, heart, lung, liver, submandibular gland, kidney, pancreas, testis, muscle and brown fat. EGFP expression can not be observed.(For more detailed information please contact our technical advisor.)

Fig. 6 The EGFP protein expression was detected in the crypt cells of intestine by immunofluorescence staining using anti-EGFP antibody.