Nos1-IRES-Cre

Nomenclature

C57BL/6Smoc-Nos1em1(IRES-iCre)Smoc

Cat. NO.

NM-KI-200113

Strain State

Sperm cryopreservation

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Gene Summary

Gene Symbol
Nos1

Synonyms

NO; NOS; bNOS; nNOS; N-NOS; NOS-I; Nos-1; NC-NOS; 2310005C01Rik

NCBI ID

18125

MGI ID

97360

Ensembl ID

ENSMUSG00000029361

Pubmed

Nos1

Human Ortholog

NOS1

Model Description

A IRES-iCre expression cassette was knocked into the Nos1 gene stop codon site.
Research Application：Cre recombinase tool; NOS1, the neurogenic nitric oxide synthase, is a key enzyme responsible for the production of the signaling molecule nitric oxide (NO) by neurons. When crossed with a strain carrying a gene flanked by loxP sites, the flanked gene will be removed in cells expressing cre. This strain may be useful for studying diseases with impaired or reduced nitric oxide release, such as achalasia of cardia, hirschsprung's disease, hypertrophic pyloric stenosis, etc.

Validation Data

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Fig. 1 Cre-mediated recombination in the brain of Nos1Cre/+; Rosa26tdTomato/+ mouse. TdTomato(red) expression can be detected in the olfactory bulb, cortex and hippocampus derived from Nos1Cre/+; Rosa26tdTomato/+ mouse.

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Fig. 2 Cre-mediated recombination in the large intestine of Nos1Cre/+; Rosa26tdTomato/+ mouse. TdTomato(red) expression can be detected in the muscular layer and mucous layer of the wall of the large intestine derived from Nos1Cre/+; Rosa26tdTomato/+ mouse.

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Fig. 3 Detection of tdTomato(red) in various tissues of Nos1Cre/+; Rosa26tdTomato/+ mice. Cre mediated recombination can be detected in  the brain, eyes, pituitary gland, heart, liver, stomach, brown fat, kidney, pancreas, thymus, colon, spleen, skin, muscle, uterus, ovary and submandibular gland. (For more detailed information please contact our technical advisor.)


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