Foxp3-IRES-tdTomato-2A-CreERT2

Nomenclature

C57BL/6JSmo-Foxp3em4(IRES-tdTomato-2A-CreERT2)Smoc

Cat. NO.

NM-KI-190120

Strain State

Repository Live

Export PDF

Gene Summary

Gene Symbol
Foxp3

Synonyms

sf; JM2; scurfin

NCBI ID

20371

MGI ID

1891436

Ensembl ID

ENSMUSG00000039521

Pubmed

Foxp3

Human Ortholog

FOXP3

Model Description

A IRES-tdTomato-2A-CreERT2 expression cassette was knocked into the Foxp3 gene stop codon site.
Research Application：Cre tool mouse

Validation Data

image.png

Fig1. Detection of tdTomato and zsGreen expressed on mCD4+ mFoxp3+ Treg cells  in Peripheral blood and spleen in WT and Foxp3CreERT2/+; Rosa26zsGreen/+ mice.

image.png


Fig2. CreERT2 mediated recombination in large intestine of Foxp3 CreERT2/+;Rosa26 zsGreen/+ mice.

image.png


Fig3. Detection of zsGreen(green) and tdTomato(red) in various tissues of Foxp3 CreERT2/+;Rosa26 zsGreen/+ mice. Tissues expressing zsGeen: large intestine, small intestine, lung, stomach, spleen, pancreas, skin, thymus, mesenteric lymph nodes. Tissues expressing zsGeen in small amounts: liver, kidney. (58-days old, male) (For more detailed information please contact our technical advisor.) 

You may also like

Tamoxifen诱导Cre-ERT2小鼠 使用指南

Cre-ERT2在无Tamoxifen诱导的情况下，在细胞质内处于无活性状态；当Tamoxifen诱导后，Tamoxifen的代谢产物4-OHT（雌激素类似物）与ERT结合，可使Cre-ERT2进核发挥Cre重组酶活性。

Learn more

【小鼠大学问】基因工程小鼠的命名规则

常见的基因工程小鼠可以分为两种命名方式，包括基因定点修饰的小鼠命名，比如：敲除、敲入、点突变等等，和随机转基因的小鼠命名。

Learn more

Cre-lox系统介绍及使用汇总

你一定听说过Cre-lox重组系统，无论你是否直接进行过基因操作。由于Cre-lox系统具有操作简单、重组率高的优点，如今已经成为体内外遗传操作的强有力工具。利用Cre-lox系统，可以在特定细胞、组织或整个生物体，甚至在特定时间点敲除或表达某个基因，实现对特定基因的时空特异性操作，这对基因功能的研究和人类疾病动物模型的建立都具有深刻影响。

Learn more