CAG-LSL-dCas9-SPH-Tg

Nomenclature

C57BL/6Smoc-Tg(CAG-LSL-dCas9-SPH)Smoc

Cat. NO.

NM-TG-00025

Strain State

Repository Live

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Model Description

Cre-dependent SunTag-p65-HSF1 (SPH) transgenic mice were generated with piggyBac transposon system in F1 zygotes and crossed with wild type C57BL/6 mice., SPH transgenic mouse containing HA-tagged dCas9 fused with 10xGCN4, which is linked with p65-HSF1 and EGFP in tandem via P2A and T2A respectively. The transgene is driven by the ubiquitous CAG promoter and interrupted by a loxP-stop-loxP (LSL) cassette to render Cas9 expression inducible by the Cre recombinase. Expression of dCas9 can be detected using primary antibody: rabbit monoclonal antibody to HA-tag (1:1000, #3724, CST45) and secondary antibody: goat anti-rabbit Alexa Fluor 488 (1:1000, #A-11034, Thermo Fisher). *This strain is a client-donated line. The donor's publication indicates that fluorescence was observed by immunofluorescence. The JAX description of this strain also explicitly states that fluorescence cannot be directly observed.
Research Application：gene regulation tool,CRISPRa

Publications

In vivo simultaneous transcriptional activation of multiple genes in the brain using CRISPR–dCas9- activator transgenic mice
References：Nature Neuroscience

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