hSNCA

Fig.1 Detection of SNCA expression in frontal lobe of 12-week-old female mice by RT-PCR. 

Wild type: one band at 153 bp with primers F1/R1(mSnca);

Heterozygous: one band at 153 bp with primers F1/R1(mSnca) and one band at 120 bp with primers F2/R2(hSNCA).

Abbr. M, DNA marker; HE, heterozygous; WT, wild type.

Fig.2 Detection of human SNCA expression in hSNCA knockin mice by WB. 

Abbr. M, marker; WT, wild type; HE, heterozygous.

Note. The primary antibody cross-reacted with mouse SNCA and humanized SNCA.

Fig.3 Detection of SNCA expression in hSNCA mice by WB. Lysates of hippocampus, cortex and heart tissues were collected from 7-week-old male wild-type C57BL/6 mice and 6 or 7-week-old male homozygous hSNCA mice, and then analyzed by western blot with anti-SNCA antibody. 

Abbr. HO, homozygous; WT, wild type; M, marker.