hBCMA

Fig.1 Detection of BCMA expression in small intestine and spleen by RT-PCR. 

Wild type: only one band at 137 bp with primers F1/R1 (mBcma);

Homozygous: only one band at 361 bp with primers F2/R2 (hBCMA).

Abbr. M, DNA marker; HO, homozygous; WT, wild type.

Fig.2 Detection of hBCMA expression on TACI+CD138+ cells in spleen of WT C57BL/6 mice and HO hBCMA mice by FACS. Leukocytes were collected from wild-type C57BL/6 mice (male, 11 weeks old) and homozygous hBCMA humanized mice (female, 12 weeks old), and analyzed by flow cytometry with anti-hBCMA. hBCMA was exclusively detectable in hBCMA humanized mice but not in wild-type mice. hBCMA was exclusively detectable on TACI+CD138+ cells in hBCMA humanized mice.

Abbr. HO, homozygous; WT, wild type.

Fig.3 Detection of hBCMA expression on TACI+CD138+ cells in bone marrow of WT C57BL/6 mice and HO hBCMA mice by FACS. Leukocytes were collected from wild-type C57BL/6 mice (male, 11 weeks old) and homozygous hBCMA humanized mice (female,12 weeks old), and analyzed by flow cytometry with anti-hBCMA. hBCMA was exclusively detectable in hBCMA humanized mice but not in wild-type mice. hBCMA was exclusively detectable on TACI+CD138+ cells in hBCMA humanized mice.

Abbr. HO, homozygous; WT, wild type.

Fig.4 Frequency of leukocyte and myeloid subpopulations in the blood by flow cytometry. Blood was collected from WT C57BL/6 mice and HO hBCMA mice (12-week-old, n=4). Flow cytometry analysis of frequencies of (A) lymphocyte and (B) myeloid subpopulations in the blood. hBCMA knockin mice showed a normal immune phenotype in blood. Bar indicated expressed as mean ± SEM.

Abbr.  HO, homozygous; WT, wild type.

Fig.5 Frequency of leukocyte and myeloid subpopulations in spleen by flow cytometry. Spleen was collected from WT C57BL/6 mice and HO hBCMA mice (12-week-old, n=3). Flow cytometry analysis of frequencies of (A) lymphocyte and (B) myeloid subpopulations in the spleen. hBCMA knockin mice showed a normal immune phenotype in spleen. Bar indicated expressed as mean ± SEM.

Abbr.  HO, homozygous; WT, wild type.