h4-1BB(2)

Figure 1. Expression of humanized CD137(4-1BB) in the activated spleen lymphocytes of heterozygous humanized 4-1BB mice is detected by FACS. 

The spleen lymphocytes of heterozygous humanized 4-1BB mice were activated by anti-CD3 and anti-CD28 for 48 hours, and then collected for staining. The expression of humanized 4-1BB was detected by FACS. The results showed that the active expression of humanized 4-1BB can be detected in both activated CD4+ and CD8+ T lymphocytes collected from heterozygous humanized 4-1BB mice. (Cooperation with CrownBio)

Figure 2  Analysis of human 4-1BB(CD137) expression in the spleen by FACS. The homozygous KI mouse expresses human 4-1BB on the CD8⁺ T cells.

Figure 3. In vivo validation of humanized 4-1BB mice. Humanized 4-1BB mice were inoculated with MC38 colon cancer cells, and were randomly assigned into different groups (n=6) when the tumors grew to a volume of 100 mm3. Human-specifc, 4-1BB antibody ( Urelumab or Utomilumab) was given every three days (Q3D) for two weeks (In collaboration with Crownbio).