hCD3EDG(BALB/c)

Nomenclature

BALB/c-Cd3etm1(hCD3E)Cd3dtm1(hCD3D)Cd3gtm1(hCD3G)/CbSmoc

Cat. NO.

NM-HU-220122

Strain State

Repository Live

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Model Description

The endogenous mouse Cd3e/Cd3d/Cd3g genes were replaced by human CD3E/CD3D/CD3G gene.

Validation Data

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Fig1. Detection of surface expression of human CD3E/CD3D on T cells in spleen in homozygous hCD3EDG (BALB/c) mice. (In collaboration with CrownBio.)

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Fig2. Detection of surface expression of human CD3E/CD3G on T cells in blood in homozygous hCD3EDG (BALB/c) mice. (In collaboration with CrownBio.)

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Fig3. Analysis of TCR signalling in hCD3EDG (BALB/c) mice upon CD3/CD28 activation in vitro. (In collaboration with CrownBio.)

Note: CFSE-dilution as a measure of mouse T cell proliferation.

CD3+ T cells were isolated in the splenocytes from WT BALB/c and hCD3EDG mice. Isolated T cells were labeled with µCFSE dye and stimulated with anti-mCD3 (5µg/mL) or anti-hCD3 (5µg/mL) plus soluble anti-mCD28 (50µg/mL) for in vitro culture for 72 hr. T cell proliferation was analyzed by flow cytometry for CFSE dilution. 

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