hCSF1/hCSF1R(BALB/c)

Nomenclature

BALB/cAnSmoc-Csf1tm2(CSF1)Csf1rtm3(hCSF1R)Smoc

Cat. NO.

NM-HU-220016

Strain State

Embryo cryopreservation

Export PDF

Model Description

The endogenous mouse Csf1 and Csf1r genes was replaced respectively by human CSF1 and CSF1R genes.

Validation Data

image.png

Fig.1 Detection of CSF1 expression in spleen and lung by RT-PCR. 

Wild type: only one band at 299 bp with primers F1/R1(mCsf1);

Homozygous: only one band at 261 bp with primers F2/R2(hCSF1); 

Abbr. M, DNA marker; HO, homozygous; WT, wild type.

image.png

Fig.2 Detection of CSF1R expression in spleen and lung by RT-PCR. 

Wild type: only one band at 233 bp with primers F1/R1(mCsf1r);

Homozygous: only one band at 345 bp with primers F2/R2(hCSF1R); 

Abbr. M, DNA marker; HO, homozygous; WT, wild type.

image.png

Fig.3 Detection of hCSF1 expression in serum by ELISA (n=3). 

Abbr. HO, homozygous; WT, wild type.

Note. hCSF1/hCSF1R and BALB/c mice were i.p. injected with LPS for 6h.

image.png

Fig.4 Expression characterization of hCSF1R on Monocytes/ macrophages in Bone marrow in hCSF1/hCSF1R dKI mice.

You may also like

Shanghai Model Organisms Center Inc has licensed CRISPR-Cas9 technology from Broad Institute

On Dec 16, 2018, Broad Institute and Shanghai Model Organisms Center Inc (SMOC) has entered into a non-exclusive license agreement under which Broad has granted SMOC worldwide rights to commercialize a service platform for genetically modified mouse models under Broad's intellectual property.

Learn more
Workshop：Progress and Advances in Preclinical immuno-Oncology Research

SMOC’s Annual Progress and Advances in Preclinical immuno-Oncology Research: The workshop is designed as a forum for ideas and opinions exchange on how to decrease the rate of clinical failures in oncology and immuno-oncology.

Learn more