hGCGR

Nomenclature

C57BL/6Smoc-Gcgrtm(hGCGR)Smoc

Cat. NO.

NM-HU-215004

Strain State

Embryo cryopreservation

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Gene Summary

Gene Symbol
Cxcr5

Synonyms

Blr1; Gpcr6; MDR15; CXC-R5; CXCR-5

Chromosome

9

NCBI ID

12145

MGI ID

103567

Ensembl ID

ENSMUSG00000047880

Pubmed

Cxcr5

Human Ortholog

CXCR5

Model Description

The endogenous mice Gcgr gene was replaced by human GCGR gene.

Validation Data

image.png

Fig.1 Detection of Mouse Gcgr expression in liver, kidney, heart, lung, pancreas, brown adipose tissue, white adipose tissue, jejumum, ileum, colon,cortex, cerebellum, hypothalamus and brain stem by RT-PCR. 

Wild type: only one band at 226 bp with primers F1/R1(mGcgr);

Homozygous: only one band at 190 bp with primers F2/R2(hGCGR).

Abbr. M, DNA marker; HO, homozygous; WT, wild type.

image.png

Fig.2 Detection of GCGR expression in liver, kidney, heart, lung, pancreas, brown adipose tissue, white adipose tissue, jejumum, ileum, colon,cortex, cerebellum, hypothalamus and brain stem by RT-PCR. 

Wild type: only one band at 226 bp with primers F1/R1(mGcgr);

Homozygous: only one band at 190 bp with primers F2/R2(hGCGR).

Abbr. M, DNA marker; HO, homozygous; WT, wild type.

image.png

Fig.3 Detection of hGCGR expression in hGCGR mice by WB.

Abbr. M,marker; HO, homozygous; WT, wild type; PC, positive control, Hela cells.

Note.The anti-GCGR antibody cross-reacted with mouse GCGR and humanized GCGR.

image.png

Fig.4 Detection of hGCGR expression in Kidney in hGCGR KI mice.

Note. The anti-human GCGR antibody cross-reacted with mouse GCGR and humanized GCGR.

image.png

Fig.5 Detection of hGCGR expression in Liver in hGCGR KI mice.

Note. The anti-human GCGR antibody cross-reacted with mouse GCGR and humanized GCGR.

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