hIL12A

Nomenclature

C57BL/6Smoc-Il12atm3(hIL12A)/Smoc

Cat. NO.

NM-HU-2000056

Strain State

Embryo cryopreservation

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Gene Summary

Gene Symbol
Il12a

Synonyms

p35; Ll12a; Il-12a; IL-12p35

NCBI ID

16159

MGI ID

96539

Ensembl ID

ENSMUSG00000027776

Pubmed

Il12a

Human Ortholog

IL12A

Model Description

The endogenous mouse Il12a gene was replaced by human IL12A gene.
Research Application：Cytokine humanization model；drug screening

Validation Data

image.png

Fig1. Detection of IL12A expression in spleen by RT-PCR. 

Wild type: only one band at 198 bp with primers F1/R1(mIl12a);

Homozygous: only one band at 488 bp with primers F2/R2(hIL12A); 

Abbr.. M, DNA marker; HO, homozygous; HE, heterozygous; WT, wild type.

image.png

Fig2. Detection of human IL12P70 and mouse IL12P70 expression in serum by ELISA (n=3). 

Abbr. HO, homozygous; WT, wild type.

Note. hIL12A/hIL12B and C57BL/6 mice were i.p. injected with LPS for 2h.

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