hC5

Nomenclature

C57BL/6Smoc-Hctm1(hC5)Smoc

Cat. NO.

NM-HU-2000013

Strain State

Repository Live

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Model Description

The endogenous mouse C5a gene was replaced by human C5 gene.
Research Application：Immunotherapy,cancer research,drug screening

Validation Data

image.png

Fig 1. Detection of C5 expression by RT-PCR. 

Wild type: only one band at 167 bp with primers F2/R2(mC5);

Homozygous: only one band at 337 bp with primers F1/R1(hC5).

Abbr. M, DNA marker; HO, homozygous; HE, heterozygous; WT, wild type.

image.png

Fig 2. Analysis of humanized C5 and humanized C5a expression in serum in the humanized C5 mouse by ELISA. 

The homozygous hC5 KI mice were treated with LPS or NS (3mg/kg, i.p.) for 6 hours.

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Fig 3. Detection of hC5 expression in eyes in hC5 KI mice by IF (n=3/group). 

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