Cd8a-2A-tdTomato-2A-Luc

Nomenclature

C57BL/6Smoc-Cd8aem(T2A-tdTomato-P2A-Luciferase)1Smoc

Cat. NO.

NM-KI-225025

Strain State

Sperm cryopreservation

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Model Description

T2A-tdTomato-P2A-Luciferase expression cassette was knocked into the Cd8a gene .
Research Application:Reporter mouse

Validation Data

Fig.1.png

Fig.1 IVIS automatic exposure. According to the imaging process, the mice were injected with D-Luciferin, and the abdomen, back, and flanks were imaged in vivo. 

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Fig.2 IVIS automatic exposure: ex vivo. According to the imaging process, D-Luciferin was injected, and the mouse brain, heart, lung, kidney, liver,  spleen, thymus, inguinal lymph node, axial lymph node (Axial LN), muscle, and the entire digestive system  (stomach to colon) were taken for imaging.

Fig.3.pngFig.3 Detection of tdTomato in mice thymus by FACS.

Fig.4.png

Fig.4 Detection of tdTomato in mice axillary lymph by FACS.

Fig.5.png

Fig.5 Detection of tdTomato in mice inguinal lymph by FACS.

Fig.6.pngFig.6 Detection of tdTomato in mice spleen by FACS.

Fig.7.pngFig.7 Detection of tdTomato in mice PB by FACS.

*The validation data above were completed in cooperation with Crownbio.


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