hPD-L2

Fig1.Schematic representation of gene targeting at the Pd-l2 locus.

Fig2. Genotyping of humanized PD-L2 mice by RT-PCR.

Primer P1/2

Wild type = 404 bp

Primer P3/4

Mutant type = 405 bp

M:100bp Plus DNA ladder from Transgen (BM311)

Fig 3. Splenocytes from humanized PD-L2 mice were analyzed by flow cytometry after activation. 

hPD-L2+ (but not mPD-L2+) cells were detectable in the homozygous hPD-L2 mice, while hPD-L2+ and mPD-L2+ cells were both detectable in heterozygous humanized PD-L2 mice.