Atp4b-IRES-CreERT2(2)

An IRES-CreERT2 expression cassette was knocked into the Atp4b gene stop codon site. Besides,An IRES-CreERT2-WPRE-pA expression cassette were knock into the Atp4b gene start codon site to generate Atp4b-IRES-CreERT2(Stock No. NM-KI-210118) mice. ATP4B could serve as a tumor suppressor in the tumorigenesis and progression. This strain is useful in studying the function of gastric parietal cells in gastric epithelial homeostasis. When crossed with a strain carrying a gene flanked by loxP sites, the flanked gene will be removed in cre-expressing cells after tamoxifen treatment.

Fig. 1 CreERT2-mediated recombination in the stomach of Atp4b^CreERT2/+; Rosa26^tdTomato/+ mouse. TdTomato(red) expression can be detected in the stomach of Atp4b^CreERT2/+; Rosa26^tdTomato/+ mouse after tamoxifen treatment. Some leakiness were detected prior to tamoxifen exposure.

Fig. 2 Detection of tdTomato(red) in various tissues of Atp4b^CreERT2/+; Rosa26^tdTomato/+ mice after tamoxifen treatment. Tdtomato was expressed in the glandular stomach and individual cells of thymus. Some leakiness were detected prior to tamoxifen exposure. TdTomato can not be detected in the brain, heart, lung, salivary gland, brown fat, kidney, pancreas, colon, intestine, spleen, ovary, muscle and skin. (For more detailed information please contact our technical advisor.)