Lgr5-2A-DreERT2

Nomenclature

C57BL/6Smoc-Lgr5em1(2A-DreERT2)Smoc

Cat. NO.

NM-KI-200178

Strain State

Sperm cryopreservation

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Gene Summary

Gene Symbol
Lgr5

Synonyms

FEX; Gpr49

NCBI ID

14160

MGI ID

1341817

Ensembl ID

ENSMUSG00000020140

Pubmed

Lgr5

Human Ortholog

LGR5

Model Description

A 2A-DreERT2 expression cassette was knocked into the Lgr5 gene stop codon site.
Research Application：Dre recombinase tool

Validation Data

image.png

Fig1. Dre mediated recombination in liver of Lgr5DreERT2/+; Rosa26tdTomato/+ mouse.

Hepatocyte expressing tdTomato(red) after tamoxifen induction of Lgr5DreERT2/+; Rosa26tdTomato/+ mouse. (see the reference). 

image.png

Fig2. Dre mediated recombination in colon of Lgr5DreERT2/+; Rosa26tdTomato/+ mouse.

Lgr5-2A-DreERT2 43T,46.jpg

Fig3. Detection of tdTomato(red) in various tissues of Lgr5DreERT2/+; Rosa26tdTomato/+ mouse. 

Tissues expressing tdTomato after tamoxifen induction included the large intestine, small intestine, liver, stomach, and skin. In contrast, no significant fluorescent expression was observed in the brain, spinal cord, esophagus, muscle, vagina, or reproductive system of the mice following tamoxifen induction. (38-days old, female) (For more detailed information please contact our technical advisor.) 


Publications

A Suite of New Dre-recombinase Drivers Markedly Expands the Ability to Perform Intersectional Genetic Targeting
References：CELL STEM CELL

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