hC1S

Nomenclature

C57BL/6JSmo-C1s1tm1(hC1S)/Smoc

Cat. NO.

NM-HU-2000077

Strain State

Embryo cryopreservation

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Gene Summary

Gene Symbol
C1s1

Synonyms

C1s; C1sa; AA959438; AI255193; AI327365

Chromosome

6

NCBI ID

50908

MGI ID

1355312

Ensembl ID

ENSMUSG00000038521

Pubmed

C1s1

Human Ortholog

C1S1

Model Description

The endogenous mouse C1s1 gene was replaced by human C1S gene.
Research Application：Immunotherapy,drug screening

Validation Data

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Fig1. Detection of C1S1 expression in bladder by RT-PCR. Wild type: only one band at 396 bp with primers F1/R1(mC1s1); Homozygous: only one band at 294 bp with primers F2/R2(hC1S1); Abbr. M, DNA marker; HO, homozygous; HE, heterozygous; WT, wild type.

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Fig2. Detection of hC1S1 expression in serum by ELISA (n=8). Abbr. HO, homozygous; WT, wild type.

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Fig3. Detection of mC3 and mC5 expression in serum hC1S1 mice by ELISA (n=3). Abbr. HO, homozygous; WT, wild type.

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Fig .4 Detection of classical complement pathway activity in hC1S mice by ELISA. 

Abbr. HO, homozygous; WT, wild type; PC, Postive Control; NC, Negative Control. Unpaired t test were used, n.s. p>0.05.

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