hGPR146

Nomenclature

C57BL/6Smoc-Gpr146em1(hGPR146)Smoc

Cat. NO.

NM-HU-233622

Strain State

Repository Live

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Gene Summary

Gene Symbol
Gpr146

Model Description

The endogenous mouse Gpr146 gene was replaced by human GPR146 gene.

Validation Data

image.png

Fig.1 Detection of GPR146 expression in liver, white and brown adipose tissue, lung, heart, and kidney by RT-PCR. 

Wild type: only one band at 339 bp with primers F1/R1(mGpr146);

Homozygous: only one band at 234 bp with primers F2/R2(hGPR146).

Abbr. M, DNA marker; HO, homozygous; WT, wild type.

image.png

Fig.2 Detection of human GPR146 expression in hGPR146 mice by WB. 

Abbr. M, marker; WT, wild type; HO, Homozygous; BAT, brown adipose tissue; PC, positive control, HCT116 cells.

Note. Arrow indicates expected molecular weight and asterisk indicates a nonspecific band.


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