hGUCY2C

Nomenclature

C57BL/6Smoc-Gucy2ctm(hGUCY2C)Smoc

Cat. NO.

NM-HU-220417

Strain State

Embryo cryopreservation

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Gene Summary

Gene Symbol
Gucy2c

Model Description

The endogenous mouse Gucy2c gene was replaced by human GUCY2C gene.

Validation Data

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Fig.1 Detection of GUCY2C expression in large intestine, small intestine and duodenum by RT-PCR. 

Wild type: only one band at 274 bp with primers F1/R1(mGucy2c);

Homozygous: only one band at 209 bp with primers F2/R2(hGUCY2C).

Abbr. M, DNA marker; HO, homozygous; WT, wild type.

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Fig.2 Detection of GUCY2C expression in large intestine, small intestine and duodenum by qPCR. 

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Fig.3 Multiple tissues of hom hGUCY2C knockin mice were stained with anti-GUCY2C antibody. 

Kidney and liver tissues served as negative controls, as the anti-human GUCY2C antibody cross-reacts with mouse and human GUCY2C. Amplifications: 200x.


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