hAPOE4

Nomenclature

C57BL/6Smoc-Apoetm2(hAPOE4)Smoc

Cat. NO.

NM-HU-190002

Strain State

Repository Live

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Gene Summary

Gene Symbol
Apoe

Synonyms

Apo-E; AI255918

Chromosome

7

NCBI ID

11816

MGI ID

88057

Ensembl ID

ENSMUSG00000002985

Pubmed

Apoe

Human Ortholog

APOE

Model Description

This APOE4 humanized mouse model was established by replacing the mouse Apoe gene coding exons and introns with the human APOE4 corresponding sequence by homologous recombination.
Research Application：Cardiovascular disease

Validation Data

Apoe4-2.png

Fig1. Genotyping of WT 、KO &  Humanized mice by PCR.

Primer P1/2 Wild type = 342 bp

Primer P3/4 mutant = 332 bp

M：100bp Plus DNA ladder.

image.png

Fig2. Detection of human APOE4 expression in Cerebellum in hAPOE4 knockin mice by IHC. 

Brian were collected from wild-type mice and HO hAPOE4 mice, and analyzed by IHC with anti-human APOE4 antibody. 

Abbr. HO, homozygous; WT, wild type.

image.png

Fig3. Detection of human APOE4 expression in Cornu ammonis 1(CA1) in hAPOE4 knockin mice by IHC. 

Brian were collected from wild-type mice and HO hAPOE4 mice, and analyzed by IHC with anti-human APOE4 antibody. 

Abbr. HO, homozygous; WT, wild type.

image.png

Fig4. Detection of human APOE4 expression in Cornu ammonis 3(CA3) in hAPOE4 knockin mice by IHC. 

Brian were collected from wild-type mice and HO hAPOE4 mice, and analyzed by IHC with anti-human APOE4 antibody. 

Abbr. HO, homozygous; WT, wild type.

image.png

Fig5. Detection of human APOE4 expression in Dentate Gyrus(DG) in hAPOE4 knockin mice by IHC. 

Brian were collected from wild-type mice and HO hAPOE4 mice, and analyzed by IHC with anti-human APOE4 antibody. 

Abbr. HO, homozygous; WT, wild type.

image.png

Fig6. Blood biochemical analysis of hAPOE4 mice (female, n=3).

image.png

Fig7. Detection of Aβ Expression in Brain Tissues of hAPOE Mice. 

No specific Aβ(6E10)-positive signals were detected. As illustrated in representative images of cortex and hippocampus, 4x

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