hAPOE4

This APOE4 humanized mouse model was established by replacing the mouse Apoe gene coding exons and introns with the human APOE4 corresponding sequence by homologous recombination.

Fig1. Genotyping of WT 、KO &  Humanized mice by PCR.

Primer P1/2 Wild type = 342 bp

Primer P3/4 mutant = 332 bp

M：100bp Plus DNA ladder.

Fig2. Detection of human APOE4 expression in Cerebellum in hAPOE4 knockin mice by IHC. 

Brian were collected from wild-type mice and HO hAPOE4 mice, and analyzed by IHC with anti-human APOE4 antibody. 

Abbr. HO, homozygous; WT, wild type.

Fig3. Detection of human APOE4 expression in Cornu ammonis 1(CA1) in hAPOE4 knockin mice by IHC. 

Brian were collected from wild-type mice and HO hAPOE4 mice, and analyzed by IHC with anti-human APOE4 antibody. 

Abbr. HO, homozygous; WT, wild type.

Fig4. Detection of human APOE4 expression in Cornu ammonis 3(CA3) in hAPOE4 knockin mice by IHC. 

Brian were collected from wild-type mice and HO hAPOE4 mice, and analyzed by IHC with anti-human APOE4 antibody. 

Abbr. HO, homozygous; WT, wild type.

Fig5. Detection of human APOE4 expression in Dentate Gyrus(DG) in hAPOE4 knockin mice by IHC. 

Brian were collected from wild-type mice and HO hAPOE4 mice, and analyzed by IHC with anti-human APOE4 antibody. 

Abbr. HO, homozygous; WT, wild type.

Fig6. Blood biochemical analysis of hAPOE4 mice (female, n=3).

Fig7. Detection of Aβ Expression in Brain Tissues of hAPOE Mice. 

No specific Aβ(6E10)-positive signals were detected. As illustrated in representative images of cortex and hippocampus, 4x