KPC

Nomenclature

C57BL/6Smoc-Trp53em4(R172H)Krasem4(LSL-G12D)Tg(Pdx1-cre)Smoc

Cat. NO.

NM-KI-210096

Strain State

Repository Live

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Gene Summary

Gene Symbol

Synonyms

ras; p21B; K-Ras; K-ras; Kras2; Ki-ras; Kras-2; K-Ras 2; c-K-ras; AI929937; c-Ki-ras，bbl; bfy; bhy; p44; p53; Tp53，Ipf1; IDX-1; IPF-1; Mody4; STF-1; pdx-1

NCBI ID

16653，22059，18609

MGI ID

96680，98834，102851

Ensembl ID

ENSMUSG00000030265，ENSMUSG00000059552，ENSMUSG00000029644

Pubmed

Human Ortholog

KRAS，TRP53，PDX1

Model Description

KPC小鼠是目前成功建立的一种胰腺导管腺癌模型小鼠，具有很多与人胰腺癌相似的特点，如胰腺内皮细胞瘤形成，强烈的免疫反应。80%的KPC小鼠出现了肝转移和肺转移的现象。KPC小鼠包含了KRAS和TP53基因突变，而在人胰腺癌的研究中发现，分别有80%和70%的患者表达这两种突变蛋白。 KPC小鼠的P53基因含有一个显性抑制性点突变（ TP53R172H ），KRAS基因含有一个条件性活化点突变（KRASG12D）。KRAS突变基因的上游含有lox-stop-lox终止序列，其在没有cre重组酶的条件下是不表达的。将Cre重组酶连接到PDX1的启动子后，其将在胰腺的腺泡、胰岛和导管中表达。在Cre介导的基因重组中，突变的KRAS基因含有的lox-stop-lox终止序列被切除，从而可在胰腺中表达KRASG12D蛋白。 通过Trp53-R172H、Kras-LSL-G12D和Pdx1-Cre-Tg交配获得。
Research Application：自发胰腺癌模型

Disease Connection

胰腺癌
Pancreatic Carcinoma

Reference(s)

Pancreatology . 2020 Jan;20(1):79-88. doi: 10.1016/j.pan.2019.11.006. Epub 2019 Nov 18.

Validation Data

验证数据-KPC小鼠模型-图1.png

图1 KPC小鼠模型的自发式性胰腺癌体，体积较大，表面不平整，多个结节状突起。

验证数据-KPC小鼠模型-图2.png

图2 KPC小鼠的胰腺癌体的HE染色结果。

胰腺细胞排列无序，组织结构呈不规则细胞团，可见胰腺导管增生，炎症细胞浸润，间质纤维形成，符合肿瘤组织结构特征。


Publications

Targeting UBE2T Potentiates Gemcitabine Efficacy in Pancreatic Cancer by Regulating Pyrimidine Metabolism and Replication Stress
References：Gastroenterology

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