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Models

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Mice

R26-CAG-LSL-hSIGLEC8-IRES-EGFP

Strain Name：C57BL/6Smoc-Gt(ROSA)26Sortm1(CAG-LSL-SIGLEC8-IRES-EGFP-WPRE-polyA)Smoc
Strain State：Embryo cryopreservation | Cat. NO.：NM-KI-210137

A CAG-LSL-SIGLEC8-IRES-EGFP-WPRE-polyA expression cassette was knocked into via the Rosa26 locus.

Mice

Hcrtr1-2A-tdTomato

Strain Name：C57BL/6Smoc-Hcrtr1em1(2A-tdTomato)Smoc
Strain State：Embryo cryopreservation | Cat. NO.：NM-KI-210125

2A-tdTomato expression cassette was knocked into the Hcrtr1 gene.

Mice

R26-CAG-LSL-SOX2-IRES-Luc-2A-EGFP

Strain Name：C57BL/6Smoc-Gt(ROSA)26Sorem(CAG-LSL-SOX2-IRES-Luciferase-2a-EGFP-WPRE-polyA)Smoc
Strain State：Sperm cryopreservation | Cat. NO.：NM-KI-210138

A CAG-LSL-SOX2-IRES-Luciferase-2a-EGFP-WPRE-polyA expression cassette was knocked into via the Rosa26 locus.

Mice

Slc12a5-IRES-EGFP

Strain Name：C57BL/6Smoc-Slc12a5em1(IRES-GFP)Smoc
Strain State：Embryo cryopreservation | Cat. NO.：NM-KI-210132

IRES-GFP expression cassette was knocked into the Slc12a5 gene.

Mice

Slc12a2-IRES-tdTomato

Strain Name：C57BL/6Smoc-Slc12a2em1(IRES-tdTomato)Smoc
Strain State：Embryo cryopreservation | Cat. NO.：NM-KI-210131

IRES-tdTomato expression cassette was knocked into the Slc12a2 gene.

Mice

R26-CAG-LSL-Avi-GFP-Rpl10a

Strain Name：C57BL/6Smoc-Gt(ROSA)26Sorem1(SA-2xpolyA-CAG-LSL-Avitag-GFP-Rpl10a-WPRE-polyA)Smoc
Strain State：Sperm cryopreservation | Cat. NO.：NM-KI-210142

A SA-2xpolyA-CAG-LSL-Avitag-GFP-Rpl10a-WPRE-polyA expression cassette was knocked into via the Rosa26 locus.

Mice

R26-CAG-LSL-Fkbp8-IRES-EGFP

Strain Name：C57BL/6Smoc-Gt(ROSA)26Sorem1(CAG-LSL-Fkbp8-IRES-EGFP-WPRE-polyA)Smoc
Strain State：Sperm cryopreservation | Cat. NO.：NM-KI-210135

A CAG-LSL-Fkbp8-IRES-EGFP-WPRE-polyA expression cassette was knocked into via the Rosa26 locus.

Mice

Tmem119-2A-tdTomato-2A-DTR

Strain Name：C57BL/6Smoc-Tmem119em1(2A-tdTomato-2A-DTR)Smoc
Strain State：Repository Live | Cat. NO.：NM-KI-210117
Validation Data：Presence

2A-tdTomato-2A-DTR expression cassette was knocked into the Tmem119 gene.

Mice

R26-SA-LSL-DTR-IRES-tdTomato

Strain Name：C57BL/6Smoc-Gt(ROSA)26Sorem1(SA-LSL-DTR-IRES-tdTomato)Smoc
Strain State：Repository Live | Cat. NO.：NM-KI-210136

A SA-LSL-DTR-IRES-tdTomato expression cassette was knocked into via the Rosa26 locus.

Mice

CAG-LSL-dCas9-SPH-Tg

Strain Name：C57BL/6Smoc-Tg(CAG-LSL-dCas9-SPH)Smoc
Strain State：Repository Live | Cat. NO.：NM-TG-00025
Publications：1piece

Cre-dependent SunTag-p65-HSF1 (SPH) transgenic mice were generated with piggyBac transposon system in F1 zygotes and crossed with wild type C57BL/6 mice., SPH transgenic mouse containing HA-tagged dCas9 fused with 10xGCN4, which is linked with p65-HSF1 and EGFP in tandem via P2A and T2A respectively. The transgene is driven by the ubiquitous CAG promoter and interrupted by a loxP-stop-loxP (LSL) cassette to render Cas9 expression inducible by the Cre recombinase. Expression of dCas9 can be detected using primary antibody: rabbit monoclonal antibody to HA-tag (1:1000, #3724, CST45) and secondary antibody: goat anti-rabbit Alexa Fluor 488 (1:1000, #A-11034, Thermo Fisher). *This strain is a client-donated line. The donor's publication indicates that fluorescence was observed by immunofluorescence. The JAX description of this strain also explicitly states that fluorescence cannot be directly observed.

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