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R26-LNL-PB

Strain Name：C57BL/6Smoc-Gt(ROSA)26Sor^{em1(CAG-LNL-PB-V5)Smoc}
Strain State：Embryo cryopreservation | Cat. NO.：NM-KI-00065

These mice harbor a loxP-flanked STOP cassette preventing transcription of a CAG promoter-driven piggyBac transposase. The targeted mutation was inserted into the Gt(ROSA)26Sor locus by homologous recombination. PiggyBac transposase is expressed when bred to mice that express Cre recombinase.

CAG-LSL-dCas9-SPH-Tg

Strain Name：C57BL/6Smoc-Tg(CAG-LSL-dCas9-SPH)Smoc
Strain State：Repository Live | Cat. NO.：NM-TG-00025

Cre-dependent SunTag-p65-HSF1 (SPH) transgenic mice were generated with piggyBac transposon system in F1 zygotes and crossed with wild type C57BL/6 mice., SPH transgenic mouse containing HA-tagged dCas9 fused with 10xGCN4, which is linked with p65-HSF1 and EGFP in tandem via P2A and T2A respectively. The transgene is driven by the ubiquitous CAG promoter and interrupted by a loxP-stop-loxP (LSL) cassette to render Cas9 expression inducible by the Cre recombinase. Expression of dCas9 can be detected using primary antibody: rabbit monoclonal antibody to HA-tag (1:1000, #3724, CST45) and secondary antibody: goat anti-rabbit Alexa Fluor 488 (1:1000, #A-11034, Thermo Fisher).

How do I establish a strain after the transgenic Founder mice are obtained?

C57BL/6J C57BL/6J mice Piggybac transposase

Gene overexpression

gene overexpression gene overexpression protocol gene overexpression techniques

Generate a mouse model in which an exogenous gene is introduced and overexpressed. An overexpression model can be used to investigate gene functions, promoter functions, or model the pathogenesis of human disease. Depending on the method to introduce foreign DNA, a random transgenes or targeted conditional overexpression model can be created.

Overview of gene editing technology

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Type of genetic engineering

genetic modified organism types of genetic engineering genetic engineering technology

Three types of genetic modification by the gene engineered technology: gene knockout. gene knock-in, gene overexpression.