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Mice

R26-CAG-LSL-dCas9-VPR-IRES-EGFP

Strain Name：C57BL/6Smoc-Gt(ROSA)26Sorem1(CAG-LSL-dCas9-VPR-IRES-EGFP-WPRE-pA)
Strain State：Repository Live | Cat. NO.：NM-KI-18004

These mice harbor a CAG-LSL-dCas9-VPR(VP64-p65-Rta)-eGFP-pA conditional expression cassette in mouse Rosa26 loci. This strain can be used in CRISPR mediated target gene transcriptional activation (CRISPRa).

Mice

Col1a1-TRE3G-dCas9-VPR-EGFP

Strain Name：C57BL/6Smoc-Col1a1em1(TRE3G-dCas9-VPR-eGFP-pA)Smoc
Strain State：Embryo cryopreservation | Cat. NO.：NM-KI-00123

These mice harbor a TRE3G-dCas9-VPR(VP64-p65-Rta)-eGFP-pA tetracycline-controlled transcriptional activation cassette in mouse Col1a1 loci. This strain can be used in CRISPR mediated target gene transcriptional activation (CRISPRa).

Mice

CAG-LSL-dCas9-SPH-Tg

Strain Name：C57BL/6Smoc-Tg(CAG-LSL-dCas9-SPH)Smoc
Strain State：Repository Live | Cat. NO.：NM-TG-00025

Cre-dependent SunTag-p65-HSF1 (SPH) transgenic mice were generated with piggyBac transposon system in F1 zygotes and crossed with wild type C57BL/6 mice., SPH transgenic mouse containing HA-tagged dCas9 fused with 10xGCN4, which is linked with p65-HSF1 and EGFP in tandem via P2A and T2A respectively. The transgene is driven by the ubiquitous CAG promoter and interrupted by a loxP-stop-loxP (LSL) cassette to render Cas9 expression inducible by the Cre recombinase. Expression of dCas9 can be detected using primary antibody: rabbit monoclonal antibody to HA-tag (1:1000, #3724, CST45) and secondary antibody: goat anti-rabbit Alexa Fluor 488 (1:1000, #A-11034, Thermo Fisher).

Mice

R26-SA-rtTA

Strain Name：B6;129S-Gt(ROSA)26Sortm1(SA-V5-M2rtTA-FRT-WPRE-FRT-polyA-attb-PGK-Neo-polyA-attp)Smoc
Strain State：Embryo cryopreservation | Cat. NO.：NM-KI-18025

These mice harbor a SA-V5-M2rtTA-FRT-WPRE-FRT-polyA-attb-PGK-Neo-polyA-attp expression cassette in mouse Rosa26 loci homologous recombination.

Mice

R26-CAG-2xKRAB-dCas9-IRES-EGFP

Strain Name：C57BL/6Smoc-Gt(ROSA)26Sorem1(CAG-Loxp-2xKRAB-dCas9-IRES-EGFP)Smoc
Strain State：Sperm cryopreservation | Cat. NO.：NM-KI-18007

These mice harbor a CAG-LSL-2xKRAB-dCas9-IRES-EGFP-WPRE-pA conditional expression cassette in mouse Rosa26 loci via homologous recombination. This strain can be used in CRISPR mediated target gene transcriptional inhibition (CRISPRi). The loxp-stop-loxp cassette was deleted.

Mice

Bpifa1-Flox

Strain Name：B6;129S-Bpifa1tm1(flox)Smoc
Strain State：Embryo cryopreservation | Cat. NO.：NM-CKO-00074

These mice carry loxP sites flanking exon 3. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific conditional expression of the gene.

Mice

Il12rb2-KO

Strain Name：C57BL/6Smoc-Il12rb2em1Smoc
Strain State：Sperm cryopreservation | Cat. NO.：NM-KO-190456

Exon 3-4 of Il12rb2 gene was deleted to generate Il12rb2 knockout mice.

Mice

Tcf7l1-KO

Strain Name：C57BL/6Smoc-Tcf7L1em1Smoc
Strain State：Sperm cryopreservation | Cat. NO.：NM-KO-190689

Exon 3 of Tcf7l1 gene was deleted to generate Tcf7l1 knockout mice.

Mice

Il1b-Luc-Tg

Strain Name：B6.Cg-Tg(IL1B-luc)Smoc
Strain State：Repository Live | Cat. NO.：NM-TG-00001

The transgenic mice model could be used to study transcriptional regulation of the IL-1βgene expression in the inflammatory process and evaluation the effect of anti-inflammatory drug in vivo.

Mice

Bmal1-Flox

Strain Name：C57BL/6Smoc-Bmal1em1(flox)Smoc
Strain State：Sperm cryopreservation | Cat. NO.：NM-CKO-200063

These mice carry loxP sites flanking exon 8 of Bmal1 gene. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific conditional expression of Bmal1 gene.

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