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Mice

Cdh1-2A-DreERT2

Strain Name：C57BL/6Smoc-Cdh1em3(2A-DreERT2)Smoc
Strain State：Sperm cryopreservation | Cat. NO.：NM-KI-190031

A 2A-DreERT2 expression cassette was knocked into the Cdh1 gene stop codon site via CRISPR/Cas mediated recombination.

Mice

Ly6g-2A-DreERT2

Strain Name：C57BL/6Smoc-Ly6gem1(2A-DreERT2)Smoc
Strain State：Sperm cryopreservation | Cat. NO.：NM-KI-190036

A 2A-DreERT2 expression cassette was knocked into the Ly6g gene stop codon site via CRISPR/Cas mediated recombination.

Mice

Tnf-IRES-Luc

Strain Name：C57BL/6Smoc-Tnfem4(IRES-Luciferase)Smoc
Strain State：Embryo cryopreservation | Cat. NO.：NM-KI-200003

A IRES-Luciferase expression cassette was knocked into the Tnf gene stop codon site via CRISPR/Cas mediated recombination.

Mice

Cyp2e1-CreERT2

Strain Name：C57BL/6Smoc-Cyp2e1em1(CreERT2)Smoc
Strain State：Sperm cryopreservation | Cat. NO.：NM-KI-00047

A kozak-CreERT2-Wpre-polyA cassette was inserted at ATG site of Cyp2e1 gene via CRISPR/Cas mediated recombination.

Mice

CAG-LSL-dCas9-SPH-Tg

Strain Name：C57BL/6Smoc-Tg(CAG-LSL-dCas9-SPH)Smoc
Strain State：Repository Live | Cat. NO.：NM-TG-00025

Cre-dependent SunTag-p65-HSF1 (SPH) transgenic mice were generated with piggyBac transposon system in F1 zygotes and crossed with wild type C57BL/6 mice., SPH transgenic mouse containing HA-tagged dCas9 fused with 10xGCN4, which is linked with p65-HSF1 and EGFP in tandem via P2A and T2A respectively. The transgene is driven by the ubiquitous CAG promoter and interrupted by a loxP-stop-loxP (LSL) cassette to render Cas9 expression inducible by the Cre recombinase. Expression of dCas9 can be detected using primary antibody: rabbit monoclonal antibody to HA-tag (1:1000, #3724, CST45) and secondary antibody: goat anti-rabbit Alexa Fluor 488 (1:1000, #A-11034, Thermo Fisher).

Mice

R26-CAG-2xKRAB-dCas9-IRES-EGFP

Strain Name：C57BL/6Smoc-Gt(ROSA)26Sorem1(CAG-Loxp-2xKRAB-dCas9-IRES-EGFP)Smoc
Strain State：Sperm cryopreservation | Cat. NO.：NM-KI-18007

These mice harbor a CAG-LSL-2xKRAB-dCas9-IRES-EGFP-WPRE-pA conditional expression cassette in mouse Rosa26 loci via homologous recombination. This strain can be used in CRISPR mediated target gene transcriptional inhibition (CRISPRi). The loxp-stop-loxp cassette was deleted.

Mice

R26-CAG-LSL-dCas9-VPR-IRES-EGFP

Strain Name：C57BL/6Smoc-Gt(ROSA)26Sorem1(CAG-LSL-dCas9-VPR-IRES-EGFP-WPRE-pA)
Strain State：Repository Live | Cat. NO.：NM-KI-18004

These mice harbor a CAG-LSL-dCas9-VPR(VP64-p65-Rta)-eGFP-pA conditional expression cassette in mouse Rosa26 loci. This strain can be used in CRISPR mediated target gene transcriptional activation (CRISPRa).

Mice

Col1a1-TRE3G-dCas9-VPR-EGFP

Strain Name：C57BL/6Smoc-Col1a1em1(TRE3G-dCas9-VPR-eGFP-pA)Smoc
Strain State：Embryo cryopreservation | Cat. NO.：NM-KI-00123

These mice harbor a TRE3G-dCas9-VPR(VP64-p65-Rta)-eGFP-pA tetracycline-controlled transcriptional activation cassette in mouse Col1a1 loci. This strain can be used in CRISPR mediated target gene transcriptional activation (CRISPRa).

Mice

Crisp3-KO

Strain Name：C57BL/6Smoc-Crisp3em1Smoc
Strain State：Embryo cryopreservation | Cat. NO.：NM-KO-205521

Exon 3-4 of Crisp3 gene was deleted to generate Crisp3 knockout mice.

Mice

Crisp2-KO

Strain Name：C57BL/6Smoc-Crisp2em1Smoc
Strain State：Embryo cryopreservation | Cat. NO.：NM-KO-205467

Exon 5-6 of Crisp2 gene was deleted to generate Crisp2 knockout mice.

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