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Mice

C57BL/6

Strain Name：C57BL/6Smoc
Strain State：Repository Live | Cat. NO.：SM-001

C57BL/6 mice have a high reproductive capacity, a long life span, and a low tumor susceptibility. Male mice are aggressive and fight frequently. Diet can cause obesity, type II diabetes, and atherosclerosis in these mice. The C57BL/6 mouse is the first to have its entire genome sequenced. It is the most extensively used inbred mouse strain as a genetic basis for congenic and mutant mice. It is also used in genetics, metabolism, immunology, neurology, behavioral research, pharmacodynamics, and medication safety evaluation.

Mice

CAG-LSL-dCas9-SPH-Tg

Strain Name：C57BL/6Smoc-Tg(CAG-LSL-dCas9-SPH)Smoc
Strain State：Repository Live | Cat. NO.：NM-TG-00025

Cre-dependent SunTag-p65-HSF1 (SPH) transgenic mice were generated with piggyBac transposon system in F1 zygotes and crossed with wild type C57BL/6 mice., SPH transgenic mouse containing HA-tagged dCas9 fused with 10xGCN4, which is linked with p65-HSF1 and EGFP in tandem via P2A and T2A respectively. The transgene is driven by the ubiquitous CAG promoter and interrupted by a loxP-stop-loxP (LSL) cassette to render Cas9 expression inducible by the Cre recombinase. Expression of dCas9 can be detected using primary antibody: rabbit monoclonal antibody to HA-tag (1:1000, #3724, CST45) and secondary antibody: goat anti-rabbit Alexa Fluor 488 (1:1000, #A-11034, Thermo Fisher).

Mice

Krtap4-6-KO

Strain Name：C57BL/6Smoc-Krtap4-6em1Smoc
Strain State：Developing | Cat. NO.：TBD

Exon 1 of Krtap4-6 gene was deleted to generate Krtap4-6 knockout mice.

Mice

Nkx2-6-KO

Strain Name：C57BL/6Smoc-Nkx2-6em1Smoc
Strain State：Developing | Cat. NO.：TBD

Exon 2 of Nkx2-6 gene was deleted to generate Nkx2-6 knockout mice.

Mice

Mill2-KO

Strain Name：C57BL/6Smoc-Mill2em1Smoc
Strain State：Embryo cryopreservation | Cat. NO.：NM-KO-200367

Exon 3-6 of Mill2 gene was deleted to generate Mill2 knockout mice.

Mice

Vegfa-KO

Strain Name：C57BL/6Smoc-Vegfaem1Smoc
Strain State：Embryo cryopreservation | Cat. NO.：NM-KO-190539

Exon 1-6 of Vegfa gene was deleted to generate Vegfa knockout mice.

Mice

Sympk-KO

Strain Name：C57BL/6Smoc-Sympkem1Smoc
Strain State：Embryo cryopreservation | Cat. NO.：NM-KO-191041

Exon 6 of Sympk gene was deleted to generate Sympk knockout mice.

Mice

G6pc-Flox

Strain Name：C57BL/6Smoc-G6pcem1(flox)Smoc
Strain State：Repository Live | Cat. NO.：NM-CKO-200053

These mice carry loxP sites flanking exon 3 of G6pc gene. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific conditional expression of G6pc gene.

Mice

Bmp1-Flox

Strain Name：C57BL/6Smoc-Bmp1em1(flox)Smoc
Strain State：Embryo cryopreservation | Cat. NO.：NM-CKO-200065

These mice carry loxP sites flanking exon 3-6 of Bmp1 gene. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific conditional expression of Bmp1 gene.

Mice

Ctnnb1-Flox

Strain Name：C57BL/6Smoc-Ctnnb1em1(flox)Smoc
Strain State：Sperm cryopreservation | Cat. NO.：NM-CKO-200154

These mice carry loxP sites flanking exon 2-6 of Ctnnb1 gene. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific conditional expression of Ctnnb1 gene.

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