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Mice

hSIRPA(2)/hCD47

Strain Name：C57BL/6Smoc-Sirpatm2(hSIRPA)Cd47em1(hCD47)/Smoc
Strain State：Repository Live | Cat. NO.：NM-HU-2000111

This SIRPA and CD47 double Knock-in strain is established by crossing hSIRPA and hCD47 mice together. These double double Knock-in mouse models can be useful for evalueting the efficacy of potential immunotherapy drug combinations.While hSIRPA/hCD47 (Stock No.NM-HU-190019) have been pulled from shelves for some reasons.

Mice

R26-CAG-LSL-mCherry-EGFP-LC3

Strain Name：C57BL/6Smoc-Gt(ROSA)26Sorem1(CAG-LSL-mCherry-EGFP-Map1lc3a-pA)Smoc
Strain State：Repository Live | Cat. NO.：NM-KI-00124

The CAG promoter-loxp-stop-loxp-mCherry-EGFP-Map1lc3a-WPRE-polyA expression cassette was inserted into the Rosa26 gene locus by homologous recombination. Map1lc3a, also known as LC3, is a widely expressed autophagic vesicle-specific marker. There was no abnormality in heterozygous mice. The presence of the loxp-stop-loxp expression cassette prevented the transcription of the downstream target gene LC3. After mating with Cre mice, LC3 gene is driven by the CAG promoter to express mCherry and EGFP in a pH-dependent manner in phagocytic cells after ischemic injury in the progeny double positive mice. The two co-expressed fluorescent signals vary depending on the acidic environment of the autophagic vesicles within the cell. mCherry is stable in an acidic environment (pKa 4.5), and quenching occurs in the acidic environment (pKa 5.9) of EGFP in lysosomes. In the autophagy vesicles with higher pH, the fluorescence of GFP and mCherry superimposed on yellow fluorescence; while in the lower pH lysosome, EGFP was quenched and only red fluorescence signal was detected. It can be used to label and track LC3 and study the origin, progression and disappearance of autophagy in various tissues after ischemic injury.

Mice

Rag1-KO(Rag1-EGFP)

Strain Name：B6;129S-Rag1tm1(loxP-EGFP-PolyA-loxP-Neo-loxP)Smoc
Strain State：Repository Live | Cat. NO.：NM-KI-00069

A loxP-EGFP-PolyA-loxP-Neo-loxP expression cassette was knocked into the Rag1 gene start codon site. As a Rag1 knockout mouse model, this stain can be used in subcutaneous inoculation of liver cancer tissues and tumor cells. Tumors can esaily form and grow. The amount of T and B lymphocytes in peripheral blood of mice was extremely low tested by FACS, which was comparable to or lower than that of Nude mice, and there was a significant difference compared with wild type mice. The pathological sections of HE staining of tumor tissues showed that the tumor sections of Rag1 KO mice and Nude mice were similar. This strain has the potential to replace Nude, NOD-SCID mice as a tumor-bearing mouse model.

Mice

Rag2-KO(Rag2-EGFP)

Strain Name：B6;129S-Rag2tm1(loxP-EGFP-PolyA-loxP-Neo-loxP)Smoc
Strain State：Embryo cryopreservation | Cat. NO.：NM-KI-00070

A loxP-EGFP-PolyA-loxP-Neo-loxP expression cassette was knocked into the Rag2 gene start codon site. As a Rag2 knockout mouse model, this stain can be used in subcutaneous inoculation of liver cancer tissues and tumor cells. Tumors can esaily form and grow. The amount of T and B lymphocytes in peripheral blood of mice was extremely low tested by FACS, which was comparable to or lower than that of Nude mice, and there was a significant difference compared with wild type mice. The pathological sections of HE staining of tumor tissues showed that the tumor sections of Rag2 KO mice and Nude mice were similar. This strain has the potential to replace Nude, NOD-SCID mice as a tumor-bearing mouse model.

Mice

Sox9-IRES-Flp

Strain Name：B6;129S-Sox9tm1(IRES-Flp)Smoc
Strain State：Embryo cryopreservation | Cat. NO.：NM-KI-00131

An IRES-Flp-polyA coexpression cassette is inserted at 3' terminal of mouse Sox9 gene via homologous recombination to establish a Sox9-Flp recombinase tool stain. This strain can be used to knockout target gene in Sox9+ cells when crossed with mice which carry frt sites flanking target gene.

Mice

Tie1-2A-Cre

Strain Name：C57BL/6Smoc-Tie1em1(2A-Cre)Smoc
Strain State：Embryo cryopreservation | Cat. NO.：NM-KI-18023

A 2A-Cre coexpression cassette is inserted befor the stop codon of mouse Tie1 gene via homologous recombination to establish a tamoxifen inducible Tie1-Cre tool stain. This strain can be used to knockout target gene in Tie1+ cells when crossed with mice which carry loxp sites flanking target gene.

Mice

Myl1-NLS-Cre

Strain Name：C57BL/6Smoc-Myl1em1(NLS-iCre-pA)Smoc
Strain State：Repository Live | Cat. NO.：NM-KI-200124

A NLS-iCre-pA expression cassette was knocked into the Myl1 gene start codon site via CRISPR/Cas9 mediated recombination.

Mice

Aplnr-2A-DreERT2

Strain Name：C57BL/6Smoc-Aplnrem1(2A-DreERT2)Smoc
Strain State：Embryo cryopreservation | Cat. NO.：NM-KI-00132

A 2A-DreERT2-SV40polyA coexpression cassette is inserted befor the stop codon of mouse Aplnr gene via homologous recombination to establish a tamoxifen inducible Aplnr-DreERT2 tool stain. This strain can be used to knockout target gene in Aplnr+ cells after tamoxifen treatment when crossed with mice which carry rox sites flanking target gene.

Mice

Zbtb46-2A-CreERT2

Strain Name：C57BL/6Smoc-Zbtb46em1(2A-CreERT2-WPRE-pA)Smoc
Strain State：Sperm cryopreservation | Cat. NO.：NM-KI-18021

A 2A-CreERT2-WPRE-pA coexpression cassette is inserted befor the stop codon of mouse Zbtb46 gene via homologous recombination to establish a tamoxifen inducible Zbtb46-CreERT2 tool stain. This strain can be used to knockout target gene in Zbtb46+ cells after tamoxifen treatment when crossed with mice which carry loxp sites flanking target gene.

Mice

Drd1-2A-CreERT2

Strain Name：C57BL/6Smoc-Drd1em1(2A-CreERT2-WPRE-pA)Smoc
Strain State：Sperm cryopreservation | Cat. NO.：NM-KI-18016

A 2A-CreERT2-WPRE-pA coexpression cassette is inserted befor the stop codon of mouse Drd1 gene via homologous recombination to establish a tamoxifen inducible Drd1-CreERT2 tool stain. This strain can be used to knockout target gene in Drd1+ cells after tamoxifen treatment when crossed with mice which carry loxp sites flanking target gene.

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