Welcome to Advanced Search

Models

About 50 results
Mice

Gad2-IRES-Cre

Strain Name：C57BL/6Smoc-Gad2em1(IRES-iCre-pA)Smoc
Strain State：Repository Live | Cat. NO.：NM-KI-200078

A IRES-iCre-pA expression cassette was knocked into the Gad2 gene stop codon site.

Mice

Nkx2-5-IRES-Cre

Strain Name：C57BL/6Smoc-Nkx2-5em1(IRES-Cre-WPRE-polyA)Smoc
Strain State：Repository Live | Cat. NO.：NM-KI-200152

A IRES-Cre-WPRE-polyA expression cassette was knocked into the Nkx2-5 gene stop codon site.

Mice

R26-CAG-LSL-Luc-tdTomato

Strain Name：C57BL/6Smoc-Gt(ROSA)26Sorem1(CAG-LSL-luci-2A-tdTomato)Smoc
Strain State：Repository Live | Cat. NO.：NM-KI-18051

A CAG-loxP STOP-loxP-luci-2A-tdTomato-WPRE-polyA cassette was knocked into the mouse ROSA26 locus . This strain of mice can be mated with mice expressing Cre recombinase so that luciferase and tdTomato fluorescent protein could be expressed for cell tracing.

Mice

R26-CAG-LSL-Avi-GFP-Rpl10a

Strain Name：C57BL/6Smoc-Gt(ROSA)26Sorem1(SA-2xpolyA-CAG-LSL-Avitag-GFP-Rpl10a-WPRE-polyA)Smoc
Strain State：Sperm cryopreservation | Cat. NO.：NM-KI-210142

A SA-2xpolyA-CAG-LSL-Avitag-GFP-Rpl10a-WPRE-polyA expression cassette was knocked into via the Rosa26 locus.

Mice

H11-CAG-LSL-mtagBFP2

Strain Name：C57BL/6Smoc-lgs2em1(CAG-LSL-mTagBFP2)Smoc
Strain State：Sperm cryopreservation | Cat. NO.：NM-KI-190006

A CAG-LSL-mTagBFP2-3xFLAG-WPRE-polyA cassette was inserted at the mouse H11 locus. When bred to a strain expressing Cre recombinase under control of various tissue specific promoters, Cre recombination deletes the LSL cassette and allows the expression of the mTagBFP2-3xFLAG protein.

Mice

R26-CAG-LSL-Fkbp8-IRES-EGFP

Strain Name：C57BL/6Smoc-Gt(ROSA)26Sorem1(CAG-LSL-Fkbp8-IRES-EGFP-WPRE-polyA)Smoc
Strain State：Sperm cryopreservation | Cat. NO.：NM-KI-210135

A CAG-LSL-Fkbp8-IRES-EGFP-WPRE-polyA expression cassette was knocked into via the Rosa26 locus.

Mice

R26-CAG-LSL-SOX2-IRES-Luc-2A-EGFP

Strain Name：C57BL/6Smoc-Gt(ROSA)26Sorem(CAG-LSL-SOX2-IRES-Luciferase-2a-EGFP-WPRE-polyA)Smoc
Strain State：Sperm cryopreservation | Cat. NO.：NM-KI-210138

A CAG-LSL-SOX2-IRES-Luciferase-2a-EGFP-WPRE-polyA expression cassette was knocked into via the Rosa26 locus.

Mice

R26-CAG-LSL-hSIGLEC8-IRES-EGFP

Strain Name：C57BL/6Smoc-Gt(ROSA)26Sorem1(CAG-LSL-SIGLEC8-IRES-EGFP-WPRE-polyA)Smoc
Strain State：Embryo cryopreservation | Cat. NO.：NM-KI-210137

A CAG-LSL-SIGLEC8-IRES-EGFP-WPRE-polyA expression cassette was knocked into via the Rosa26 locus.

Mice

R26-SA-LSL-DTR-IRES-tdTomato

Strain Name：C57BL/6Smoc-Gt(ROSA)26Sorem1(SA-LSL-DTR-IRES-tdTomato)Smoc
Strain State：Repository Live | Cat. NO.：NM-KI-210136

A SA-LSL-DTR-IRES-tdTomato expression cassette was knocked into via the Rosa26 locus.

Mice

CAG-LSL-dCas9-SPH-Tg

Strain Name：C57BL/6Smoc-Tg(CAG-LSL-dCas9-SPH)Smoc
Strain State：Repository Live | Cat. NO.：NM-TG-00025

Cre-dependent SunTag-p65-HSF1 (SPH) transgenic mice were generated with piggyBac transposon system in F1 zygotes and crossed with wild type C57BL/6 mice., SPH transgenic mouse containing HA-tagged dCas9 fused with 10xGCN4, which is linked with p65-HSF1 and EGFP in tandem via P2A and T2A respectively. The transgene is driven by the ubiquitous CAG promoter and interrupted by a loxP-stop-loxP (LSL) cassette to render Cas9 expression inducible by the Cre recombinase. Expression of dCas9 can be detected using primary antibody: rabbit monoclonal antibody to HA-tag (1:1000, #3724, CST45) and secondary antibody: goat anti-rabbit Alexa Fluor 488 (1:1000, #A-11034, Thermo Fisher). *This strain is a client-donated line. The donor's publication indicates that fluorescence was observed by immunofluorescence. The JAX description of this strain also explicitly states that fluorescence cannot be directly observed.

Email Back to top