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Crisp1-Flox

Strain Name：C57BL/6Smoc-Crisp1^{em1(flox)Smoc}
Strain State：Developing | Cat. NO.：TBD

These mice carry loxP sites flanking target exons of Crisp1 gene. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific conditional expression of Crisp1 gene.

CAG-LSL-dCas9-SPH-Tg

Strain Name：C57BL/6Smoc-Tg(CAG-LSL-dCas9-SPH)Smoc
Strain State：Repository Live | Cat. NO.：NM-TG-00025

Cre-dependent SunTag-p65-HSF1 (SPH) transgenic mice were generated with piggyBac transposon system in F1 zygotes and crossed with wild type C57BL/6 mice., SPH transgenic mouse containing HA-tagged dCas9 fused with 10xGCN4, which is linked with p65-HSF1 and EGFP in tandem via P2A and T2A respectively. The transgene is driven by the ubiquitous CAG promoter and interrupted by a loxP-stop-loxP (LSL) cassette to render Cas9 expression inducible by the Cre recombinase. Expression of dCas9 can be detected using primary antibody: rabbit monoclonal antibody to HA-tag (1:1000, #3724, CST45) and secondary antibody: goat anti-rabbit Alexa Fluor 488 (1:1000, #A-11034, Thermo Fisher). *This strain is a client-donated line. The donor's publication indicates that fluorescence was observed by immunofluorescence. The JAX description of this strain explicitly states that fluorescence cannot be directly observed.

R26-CAG-LSL-Armc12-IRES-tdTomato

Strain Name：C57BL/6Smoc-Gt(ROSA)26Sor^{em1(CAG-LSL-Armc12-IRES-tdTomato-Wpre-pA)Smoc}
Strain State：Embryo cryopreservation | Cat. NO.：NM-KI-200290

A CAG-LSL-Armc12-IRES-tdTomato-Wpre-pA expression cassette was knocked into the Rosa26 locus via CRISPR/Cas9 mediated recombination.

R26-CAG-LSL-hFCGR3A-IRES-tdTomato

Strain Name：C57BL/6Smoc-Gt(ROSA)26Sor^{em1(SA-FRT-2xpolyA-CAG-FRT-loxp-stop-loxp-FCGR3A-IRES-tdTomato-WPRE-polyA)Smoc}
Strain State：Embryo cryopreservation | Cat. NO.：NM-KI-200255

A SA-FRT-2xpolyA-CAG-FRT-loxp-stop-loxp-FCGR3A-IRES-tdTomato-WPRE-polyA expression cassette was knocked into the Rosa26 locus via CRISPR/Cas9 mediated recombination.

R26-CAG-LSL-Kras*G12C-IRES-Luc(BALB/c)

Strain Name：BALB/cAnSmoc-Gt(ROSA)26Sor^{em2(CAG-FRT-LSL-Kras(G12C)-IRES-Luciferase-polyA)Smoc}
Strain State：Sperm cryopreservation | Cat. NO.：NM-KI-200324

A CAG-FRT-loxp-stop-loxp-Kras(G12C)-IRES-Luciferase-polyA expression cassette was knocked into the R26 locus via CRISPR/Cas9 mediated recombination.

Tigre-pTRE2-LSL-ZGX!EmGFP-2A-ZGX!TagGFP2-2A-ZGX!hrGFP-CAG-LSL-tTA2

Strain Name：C57BL/6Smoc-Igs7^{em1(2Xlns-pTRE2-Lox2272-stop-Lox2272-ZGX!emGFP-T2A-ZGX!TagGFP2-P2A-ZGX!hrGFP-Wpre-pA-2Xlns-pCAG-LoxP-stop-LoxP-tTA2-Wpre-pA)Smoc}
Strain State：Embryo cryopreservation | Cat. NO.：NM-KI-200288

A 2Xlns-pTRE2-Lox2272-stop-Lox2272-ZGX!EmGFP-T2A-ZGX!TagGFP2-P2A-ZGX!hrGFP- Wpre-pA-2Xlns-pCAG-LoxP-stop-LoxP-tTA2-Wpre-pA expression cassette was knocked into the TIGRE locus via CRISPR/Cas9 mediated recombination.

F8-KO(SD)

Strain Name：SD-F8^em1Smoc Strain State：Repository Live | Cat. NO.：NR-KO-200003

Using CRISPR/Cas9 technology, exon 16 of F8 gene was deleted to generate F8 knockout rat.

Foxn1-KO(SD)

Strain Name：SD-Foxn1^em1Smoc Strain State：Embryo cryopreservation | Cat. NO.：NR-KO-190002

Using CRISPR/Cas9 technology, Exon 2-4 of Foxn1 gene was deleted to generate Foxn1 knockout rat.

CRISPR/Cas9-mediated somatic and germline gene correction to restore hemostasis in hemophilia B mice

Model Organisms：小鼠 Product & Service：F9 Research field：hemophilia

CRISPR/Cas9‑mediated somatic and germline gene correction to restore hemostasis in hemophilia B mice

Model Organisms：Mouse Product & Service：F9 Research field：Hemophilia