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Mice

Rps4l-Flox(AI)

Strain Name：C57BL/6Smoc-Rps4lem2(flox)Smoc
Strain State：Sperm cryopreservation | Cat. NO.：NM-CKO-242022

The artificial intron was knocked into the Rps4l gene exon 1. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific conditional expression of Rps4l gene. Construction strategy References CRISPR-Cas9-mediated insertion of a short artificial intron for the generation of conditional alleles in mice.

Mice

Crisp4-KO

Strain Name：C57BL/6Smoc-Crisp4em1Smoc
Strain State：Developing | Cat. NO.：TBD

Exon 3 of Crisp4 gene was deleted to generate Crisp4 knockout mice.

Mice

Crisp3-KO

Strain Name：C57BL/6Smoc-Crisp3em1Smoc
Strain State：Embryo cryopreservation | Cat. NO.：NM-KO-205521

Exon 3-4 of Crisp3 gene was deleted to generate Crisp3 knockout mice.

Mice

Crisp2-KO

Strain Name：C57BL/6Smoc-Crisp2em1Smoc
Strain State：Embryo cryopreservation | Cat. NO.：NM-KO-205467

Exon 5-6 of Crisp2 gene was deleted to generate Crisp2 knockout mice.

Mice

Crisp1-KO

Strain Name：C57BL/6Smoc-Crisp1em1Smoc
Strain State：Developing | Cat. NO.：TBD

Exon 3-4 of Crisp1 gene was deleted to generate Crisp1 knockout mice.

Mice

Crisp3-Flox

Strain Name：C57BL/6Smoc-Crisp3em1(flox)Smoc
Strain State：Developing | Cat. NO.：NM-CKO-260797

These mice carry loxP sites flanking exon3-4 of Crisp3 gene. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific conditional expression of Crisp3 gene.

Mice

Crisp4-Flox

Strain Name：C57BL/6Smoc-Crisp4em1(flox)Smoc
Strain State：Developing | Cat. NO.：TBD

These mice carry loxP sites flanking target exons of Crisp4 gene. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific conditional expression of Crisp4 gene.

Mice

Crisp2-Flox

Strain Name：C57BL/6Smoc-Crisp2em1(flox)Smoc
Strain State：Developing | Cat. NO.：TBD

These mice carry loxP sites flanking target exons of Crisp2 gene. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific conditional expression of Crisp2 gene.

Mice

Crisp1-Flox

Strain Name：C57BL/6Smoc-Crisp1em1(flox)Smoc
Strain State：Developing | Cat. NO.：TBD

These mice carry loxP sites flanking target exons of Crisp1 gene. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific conditional expression of Crisp1 gene.

Mice

CAG-LSL-dCas9-SPH-Tg

Strain Name：C57BL/6Smoc-Tg(CAG-LSL-dCas9-SPH)Smoc
Strain State：Repository Live | Cat. NO.：NM-TG-00025
Publications：1piece

Cre-dependent SunTag-p65-HSF1 (SPH) transgenic mice were generated with piggyBac transposon system in F1 zygotes and crossed with wild type C57BL/6 mice., SPH transgenic mouse containing HA-tagged dCas9 fused with 10xGCN4, which is linked with p65-HSF1 and EGFP in tandem via P2A and T2A respectively. The transgene is driven by the ubiquitous CAG promoter and interrupted by a loxP-stop-loxP (LSL) cassette to render Cas9 expression inducible by the Cre recombinase. Expression of dCas9 can be detected using primary antibody: rabbit monoclonal antibody to HA-tag (1:1000, #3724, CST45) and secondary antibody: goat anti-rabbit Alexa Fluor 488 (1:1000, #A-11034, Thermo Fisher). *This strain is a client-donated line. The donor's publication indicates that fluorescence was observed by immunofluorescence. The JAX description of this strain also explicitly states that fluorescence cannot be directly observed.

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