Fibrosis
Principle The ligation of the common bile duct is used to mimic a mouse model of liver fibrosis induced by biliary obstruction
Sample requirements Male C57 mice of 7-8 weeks old
Service cycle 18 business days
Technical indicators Testing services include 1) Serum testing: biochemistry (ALT, AST, HA, ALB); 2) Pathological testing: HE and MASSON staining; 3) Detection of mRNA level: Real-time PCR; 4) Detection of protein level: Western blot, FACS.
Figure 1. Schematic diagram of bile duct ligation
Principle Carbon tetrachloride can cause damage to the liver and hence induce liver fibrosis (cirrhosis)
Sample requirements Male C57 mice of 8-10 weeks old
Service cycle Adaptation for 3 days, followed by continuous drug administration of 3, 6, 9, 12, and 16 weeks. The degree of liver fibrosis is different depending on the period of drug administration, which can be adjusted according to specific experimental requirements.
Technical indicators Testing services include 1) Serum testing: biochemistry (ALT, AST, HA, ALB), ELISA (ASPH); 2) Pathological testing: HE and MASSON staining; 3) Detection of mRNA level: Real-time PCR; 4) Detection of protein level: Western blot, FACS.
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