Tmem119-2A-CreERT2

Nomenclature

C57BL/6Smoc-Tmem119em2(P2A-CreERT2)Smoc

Cat. NO.

NM-KI-200234

Strain State

Repository Live

Export PDF

Gene Summary

Gene Symbol
Tmem119

Synonyms

obif; AW208946; BC025600

NCBI ID

231633

MGI ID

2385228

Ensembl ID

ENSMUSG00000054675

Pubmed

Tmem119

Human Ortholog

TMEM119

Model Description

将P2A-CreERT2共表达结构插入到小鼠Tmem119基因终止密码子处。
Research Application：Cre工具鼠；小胶质细胞

Validation Data

图片4-1.png

图片5.png图片6.png图片7.png图1 Tmem119-CreERT2小鼠在小胶质细胞中具有高表达。

A. 经Tamoxifen和玉米油分别处理，荧光检测Tmem119-CreER；R26-tdTomato小鼠中tdTomato的表达情况。

B. 荧光检测Tmem119-CreER；R26-tdTomato小鼠IBA1 阳性细胞中有tdTomato的表达。

C. 荧光检测Tmem119-CreER；R26-tdTomato小鼠小胶质细胞（Tmem119，Tdtomato）与星形胶质细胞（GFAP, green）共定位。

D. 荧光检测Tmem119-CreER；R26-tdTomato小鼠小胶质细胞（Tmem119，Tdtomato）与神经元（NeuN, green）共定位。

（Scale bar=10μm Hippo: Hippocampus; Str: Striatum; Sn: Substantia nigra.)

image.png

表1 Tmem119-CreERT2小鼠标记的小胶质细胞的定量分析。

Publications

Noncanonical contribution of microglial transcription factor NR4A1 to post-stroke recovery through TNF mRNA destabilization
References：PLOS BIOLOGY

Nuclear microRNA-mediated transcriptional control determines adult microglial homeostasis and brain function
References：Cell Reports

You may also like

Shanghai Model Organisms Center Inc has licensed CRISPR-Cas9 technology from Broad Institute

On Dec 16, 2018, Broad Institute and Shanghai Model Organisms Center Inc (SMOC) has entered into a non-exclusive license agreement under which Broad has granted SMOC worldwide rights to commercialize a service platform for genetically modified mouse models under Broad's intellectual property.

Learn more
Customizing Mouse Models: Delivering in 100 Days to Speed Up Your Research!

At GenoBioTX, we understand that the lengthy wait times for gene-modified mouse models can hinder your research progress. Traditional methods often require 6-9 months, leading to delays and increased costs. That’s why we’re thrilled to introduce our innovative service designed to streamline this process and deliver results faster.

Learn more