Rag2-KO

Nomenclature

C57BL/6Smoc-Rag2em2Smoc

Cat. NO.

NM-KO-190429

Strain State

Repository Live

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Gene Summary

Gene Symbol
Rag2

Synonyms

Rag-2

NCBI ID

19374 

MGI ID

97849

Ensembl ID

ENSMUSG00000032864

Pubmed

Rag2

Human Ortholog

RAG2

Model Description

敲除Rag2基因exon 3部分编码序列，从而造成移码功能缺失，建立Rag2基因敲除小鼠模型。
Research Application：免疫缺陷，荷瘤模型

Disease Connection

溃疡性结肠炎
Ulcerative Colitis

Phenotype(s)

MGI:4360989
Note：该品系需与Tbx21-KO(NM-KO-190157)交配才可能获得预期表型

Reference(s)

Garrett WS, Lord GM, Punit S, Lugo-Villarino G, Mazmanian SK, Ito S, Glickman JN, Glimcher LH, Communicable ulcerative colitis induced by T-bet deficiency in the innate immune system. Cell. 2007 Oct 5;131(1):33-45

重度联合免疫缺陷，常染色体隐性遗传，T细胞阴性，B细胞阴性，Nk细胞阳性
Severe Combined Immunodeficiency, Autosomal Recessive, T Cell-Negative, B Cell-Negative, Nk Cell-Positive

Phenotype(s)

MGI:2174910

Reference(s)

Shinkai Y, Rathbun G, Lam KP, Oltz EM, Stewart V, Mendelsohn M, Charron J, Datta M, Young F, Stall AM, Alt FW, RAG-2-deficient mice lack mature lymphocytes owing to inability to initiate V(D)J rearrangement. Cell. 1992 Mar 6;68(5):855-67

Validation Data

T、B细胞FACS检测.png

图1. Rag2(-/-)小鼠脾脏和外周血中的T 、B细胞缺失。

取Rag2(-/-)小鼠的外周血和脾脏细胞，并通过FACS对其中T 、B细胞的组成进行分析。

Publications

E4BP4-mediated inhibition of T follicular helper cell differentiation is compromised in autoimmune diseases
References：The Journal of Clinical Investigation

Induction of Foxp3 and activation of Tregs by HSP gp96 for treatment of autoimmune diseases
References：iScience

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