HLA-A24.2/B2M(2)

Fig.1 Detection of HLA-A24.2/B2M expression in spleen and liver by RT-PCR. 

Spleen and liver RNA were extracted from wild-type C57BL/6 mice (WT) and homozygous hHLA-A24.2/hB2M(2) knockin mice (HO) (n=2, male, 9 weeks old), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mRNA primers. Mouse B2m mRNA (297 bp) was detectable only in wild-type C57BL/6 mice. Human HLA-A24.2 (624bp) and human B2M mRNA (158 bp) was detectable only in homozygous hHLA-A24.2/hB2M(2) knockin mice but not in wild-type mice. 

Fig.2 Expression analysis of hB2M(A) and HLA-ABC(B) on CD45+ cells in blood of HLA-A24.2/B2M humanized mice. 

Leukocytes were collected from wild-type C57BL/6 mice(11 weeks old)  and homozygous HLA-A11.1/B2M humanized mice(13.4 weeks old) , and analyzed by flow cytometry with anti-mB2M, anti-hB2M, anti-mH2D and anti-HLA-ABC. hB2M and HLA-ABC were exclusively detectable in HLA-A24.2/B2M humanized mice, whereas mB2M and mH2D were exclusively detectable in wild-type C57BL/6 mice.

Fig.3 Detection of immune cell subpopulations in the blood of male mice by FACS (n=3 in all groups, 13 weeks). 

Abbr.  WT, wild type; HO, homozygous.

Fig.4 Detection of immune cell subpopulations in the blood of female mice by FACS (n=3 in all groups, 13 weeks). 

Abbr.  WT, wild type; HO, homozygous.

Fig.5 Detection of immune cell subpopulations in the spleen of male mice by FACS (n=3 in all groups, 13 weeks). 

Abbr.  WT, wild type; HO, homozygous.

Fig.6 Detection of immune cell subpopulations in the spleen of female mice by FACS (n=3 in all groups, 13 weeks). 

Abbr.  WT, wild type; HO, homozygous.