Tnf-KO

Nomenclature

C57BL/6Smoc-Tnfem1Smoc

Cat. NO.

NM-KO-00100

Strain State

Repository Live

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Gene Summary

Gene Symbol
Tnf

Synonyms

DIF; Tnfa; TNF-a; TNFSF2; Tnlg1f; Tnfsf1a; TNFalpha; TNF-alpha

NCBI ID

21926

MGI ID

104798

Ensembl ID

ENSMUSG00000024401

Pubmed

Tnf

Human Ortholog

TNF

Model Description

Exon2-3 of Tnf gene were targeted to generate the Tnf knockout mice.
Research Application：Immune and cancer research

Validation Data

image.png

Fig.1 Detection of TNF expression in spleen and thymus by RT-PCR. 

Wild type: only one band at 209 bp with primers F1/R1(mTnf). 

Abbr. M, DNA marker;  HO, homozygous; WT, wild type.

image.png

Fig.2 Detection of mTnf expression in spleen and thymus by Q-PCR. 

image.png

Fig.3 Detection of mTNF-a expression in serum by ELISA (male, 8wks, n=2). 

Abbr. HO, Homozygous; WT, wild type.

Note.TNF-KO and C57BL/6 mice were i.p. injected with LPS.

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