hCA9

Nomenclature

C57BL/6Smoc-Car9tm1(hCA9)Smoc

Cat. NO.

NM-HU-234371

Strain State

Embryo cryopreservation

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Gene Summary

Gene Symbol
Car9

Model Description

The endogenous mouse Car9 gene was replaced by human CA9 gene.

Validation Data

image.png

Fig.1 Detection of CA9 expression in stomach by RT-PCR. 

Wild type: only one band at 144 bp with primers F1/R1(mCar9);

Homozygous: only one band at 186 bp with primers F2/R2(hCA9).

Abbr. M, DNA marker; HO, homozygous; WT, wild type.

image.png

Fig.2 Detection of human CA9 expression in hCA9 knockin mice by WB. 

Abbr. M, marker; WT, wild type; HO, Homozygous; PC, positive control, HCT116 cells.

Note. The anti-human CA9 antibody cross-reacted with mouse CA9 and humanized CA9. Arrow indicates expected molecular weight and asterisk indicates a nonspecific band.


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