hLILRB4

Nomenclature

C57BL/6Smoc-Lilrb4atm1(hLILRB4 )Smoc

Cat. NO.

NM-HU-210024

Strain State

Repository Live

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Gene Summary

Gene Symbol
Lilrb4a

Model Description

The endogenous mice Lilrb4a gene was completely or partially replaced by human LILRB4 gene via CRISPR/Cas9 mediated recombination.
Research Application:inmmue therapy;drug screening

Validation Data

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Fig.1 Expression analysis of hLILRB4 on myeloid cells in blood of hLILRB4 humanized mice.  

Leukocytes were collected from blood of wild-type C57BL/6 mice (male, 7 weeks) and homozygous hLILRB4 mice (7 weeks, female) , and analyzed by flow cytometry with anti-hLILRB4 and anti-mLILRB4. hLILRB4 were exclusively detectable in hLILRB4 humanized mice but not in wild-type mice.

image.png

Fig.2 Expression analysis of hLILRB4 in spleen of hLILRB4 humanized mice.

Leukocytes were collected from spleen of wild-type C57BL/6 mice (male, 7 weeks) and homozygous hLILRB4 mice (7 weeks, female) , and analyzed by flow cytometry with anti-hLILRB4 and anti-mLILRB4. hLILRB4 were exclusively detectable in hLILRB4 humanized mice but not in wild-type mice.

image.png

Fig.3 Expression analysis of hLILRB4 in bone marrow (BM) of hLILRB4 humanized mice. 

Leukocytes were collected from BM of wild-type C57BL/6 mice (male, 7 weeks) and homozygous hLILRB4 mice (7 weeks, female) , and analyzed by flow cytometry with anti-hLILRB4 and anti-mLILRB4. hLILRB4 were exclusively detectable in hLILRB4 humanized mice but not in wild-type mice.


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