Rag1-KO(Rag1-EGFP)

Nomenclature

B6;129S-Rag1^{tm1(loxP-EGFP-PolyA-loxP-Neo-loxP)Smoc}

Cat. NO.

NM-KI-00069

Strain State

Repository Live

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Gene Summary

Gene Symbol
Rag1

Synonyms

Rag-1

NCBI ID

MGI ID

Ensembl ID

Pubmed

Human Ortholog

RAG1

Model Description

A loxP-EGFP-PolyA-loxP-Neo-loxP expression cassette was knocked into the Rag1 gene start codon site. As a Rag1 knockout mouse model, this stain can be used in subcutaneous inoculation of liver cancer tissues and tumor cells. Tumors can esaily form and grow. The amount of T and B lymphocytes in peripheral blood of mice was extremely low tested by FACS, which was comparable to or lower than that of Nude mice, and there was a significant difference compared with wild type mice. The pathological sections of HE staining of tumor tissues showed that the tumor sections of Rag1 KO mice and Nude mice were similar. This strain has the potential to replace Nude, NOD-SCID mice as a tumor-bearing mouse model.

Research Application：Immunodeficiency,tumor-bearing model

*Literature published using this strain should indicate: Rag1-KO(Rag1-EGFP) mice (Cat. NO. NM-KI-00069) were purchased from Shanghai Model Organisms Center, Inc..

Validation Data

Fig.1 Construction strategy of Rag1-KO.

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Fig.2 Loss of T and B cells in peripheral blood of Rag1-KO and Rag2-KO mice.

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Fig.3 Loss of T and B cells in Spleen of Rag1-KO and Rag2-KO mice.

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Fig.4 Loss of T and B cells in thymus of Rag1-KO and Rag2-KO mice.

Fig.5 Detection of EGFP expression in CD45+ cells in spleen, blood, thymus, and bone marrow of 8.7-week-old female WT and HO Rag1-KO(Rag1-EGFP) mice.