R26-CAG-Cas9

Nomenclature

C57BL/6Smoc-Gt(ROSA)26Sortm1(CAG-Cas9)Smoc

Cat. NO.

NM-KI-00120

Strain State

Repository Live

Export PDF

Gene Summary

Gene Symbol
Gt(ROSA)26Sor

Model Description

These mice were established by crossing R26-(CAG-LSL-Cas9) and EIIA mice together.
Research Application:gene editing, gene modification

Validation Data


image.png

Fig1. Analysis of Cas9 expression profile by Western Blot. The CAG-Cas9 KI mice express Cas9 in heart, liver, spleen, lung, kidney, stomach, cortex, cerebellum, testis and muscle.

J***: Cas9 KI mice from other peers 

CAG-Cas9: R26-CAG-Cas9 mice (NM-KI-00120) obtained by R26-CAG-LNL-Cas9(NM-KI-00038)  crossed with Ella-Cre

Cas9-krab: R26-CAG-3XKRAB-dCas9-IRES-EGFP(NM-KI-18007)

image.png

Fig2. Detection of CAS9 expression in R26-CAG-CAS9 mice by WB. 

Abbr. M, marker; WT, wild type; HO, homozygous; WAT, white adipose tissue; BAT, brown adipose tissue. 

Note. 60 μg of total protein were loaded for BAT and skeletal muscle, and 20 μg for other tissues.

image.png

Fig.3 Detection of CAS9 expression in T cells in R26-CAG-CAS9 mice by WB. 

Abbr. M, marker; WT, wild type; HO, homozygous.


You may also like

Shanghai Model Organisms Center Inc has licensed CRISPR-Cas9 technology from Broad Institute

On Dec 16, 2018, Broad Institute and Shanghai Model Organisms Center Inc (SMOC) has entered into a non-exclusive license agreement under which Broad has granted SMOC worldwide rights to commercialize a service platform for genetically modified mouse models under Broad's intellectual property.

Learn more
Customizing Mouse Models: Delivering in 100 Days to Speed Up Your Research!

At GenoBioTX, we understand that the lengthy wait times for gene-modified mouse models can hinder your research progress. Traditional methods often require 6-9 months, leading to delays and increased costs. That’s why we’re thrilled to introduce our innovative service designed to streamline this process and deliver results faster.

Learn more