Sarm1-KO
Nomenclature
C57BL/6JSmo-Sarm1em1Smoc
Cat. NO.
NM-KO-226267
Strain State
Repository Live
Gene Summary
Gene Symbol
Sarm1
Model Description
Validation Data
Fig.1 Detection of Sarm1 expression in cerebellum and frontal lobe by RT-PCR. Cerebellum and frontal lobe RNA was extracted from 9-year-old male wild-type C57BL/6 (WT) (n=2) and 16-year-old female homozygous Sarm1-KO mice (HO) (n=2), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mRNA primers. Mouse Gapdh mRNA (123 bp) was detectable both in wild-type C57BL/6 mice and homozygous Sarm1-KO mice. Mouse Sarm1 mRNA (129 bp) was detectable only in wild-type mice but not in homozygous Sarm1-KO mice.
Fig.2 Detection of Sarm1 expression in cerebellum and frontal lobe by qPCR. Cerebellum and frontal lobe RNA were extracted from 9-year-old male wild-type C57BL/6 (WT) (n=2) and 16-year-old female homozygous Sarm1-KO mice (HO) (n=2), then cDNA libraries were synthesized by reverse transcription, followed by qPCR with mouse Sarm1 and mouse Gapdh primers. Relative expression represents the mouse Sarm1 mRNA level relative to its average expression in WT mice . Mouse Sarm1 mRNA was detectable only in wild-type mice but not in homozygous Sarm1-KO mice.
You may also like
On Dec 16, 2018, Broad Institute and Shanghai Model Organisms Center Inc (SMOC) has entered into a non-exclusive license agreement under which Broad has granted SMOC worldwide rights to commercialize a service platform for genetically modified mouse models under Broad's intellectual property.
Learn moreSMOC’s Annual Progress and Advances in Preclinical immuno-Oncology Research: The workshop is designed as a forum for ideas and opinions exchange on how to decrease the rate of clinical failures in oncology and immuno-oncology.
Learn more
