Siglece-KO

Nomenclature

C57BL/6JSmo-Sigleceem1Smoc

Cat. NO.

NM-KO-200483

Strain State

Sperm cryopreservation

Export PDF

Gene Summary

Gene Symbol
Siglece

Synonyms

Cd170; Siglec5; Siglec9; Siglec12; Siglecl1; mSiglec-E

Chromosome

7

NCBI ID

83382

MGI ID

1932475

Ensembl ID

ENSMUSG00000030474

Pubmed

Siglece

Human Ortholog

SIGLEC9

Model Description

Exon 2-5 of Siglece gene was deleted to generate Siglece knockout mice.
Research Application：Therapeutic antibody target

Validation Data

image.png

Fig.1 Detection of siglece expression in spleen by RT-PCR. 

Wild type: one band at 392 bp with primers F1/R1(mSiglece) and one band at 366 bp with primers F2/R2(mSiglece).

Abbr. M, DNA marker;  WT, wild type； HO, homozygous.

image.png

image.png

Fig.2 Detection of siglece expression in spleen by FACS.

WT mice highly expressed Siglec-E on neutrophils, with less expression on other myeloid cells, but barely on lymphocytes, whereases Siglece-KO mice lacked expression of Siglec-E on all these immune cells.

You may also like

Shanghai Model Organisms Center Inc has licensed CRISPR-Cas9 technology from Broad Institute

On Dec 16, 2018, Broad Institute and Shanghai Model Organisms Center Inc (SMOC) has entered into a non-exclusive license agreement under which Broad has granted SMOC worldwide rights to commercialize a service platform for genetically modified mouse models under Broad's intellectual property.

Learn more
Workshop：Progress and Advances in Preclinical immuno-Oncology Research

SMOC’s Annual Progress and Advances in Preclinical immuno-Oncology Research: The workshop is designed as a forum for ideas and opinions exchange on how to decrease the rate of clinical failures in oncology and immuno-oncology.

Learn more