hCD3EDG/hEpCAM

Nomenclature

C57BL/6Smoc-Cd3etm1(hCD3E)Cd3dtm1(hCD3D)Cd3gtm1(hCD3G)Epcamtm1(hEPCAM)Smoc

Cat. NO.

NM-HU-220123

Strain State

Repository Live

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Model Description

hCD3EDG(NM-HU-220120) mice were crossed with hEpCAM(NM-HU-210032) mice to generate hCD3EDG/hEpCAM mice.

Validation Data

Table.1 Blood routine examination in male hCD3EDG/hEpCAM mice.

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Table.2 Blood routine examination in female hCD3EDG/hEpCAM mice.

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Fig.1 Detection of lymphcytes population in the (A) peripheral blood and (B) spleen of 8-week-old male and female homozygous hCD3EDG/hEpCAM dKI mice by FACS (n=3). 

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Fig.2 Pathological analysis of 8-week-old female hCD3EDG/hEpCAM dKI mice by H＆E staining. 

There were no obvious pathological changes in these tissues.

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Fig.3 Pathological analysis of 8-week-old male hCD3EDG/hEpCAM dKI mice by H＆E staining. 

There were no obvious pathological changes in these tissues.

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Fig.4 Detection of mouse EpCAM expression in 8-week-old wild-type mice (WT) by IHC with an anti-mouse EpCAM antibody (Abcam, ab221552). 

The arrows indicate the positive mouse EpCAM staining (brown) in each tissue. (40x Magnification) 

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Fig.5 Detection of human EpCAM expression in 8-week-old wild-type mice (WT) and homozygous hCD3EDG/hEpCAM knockin mice by IHC with an anti-human EpCAM antibody (Abcam, ab223582). 

The arrows indicate the positive EpCAM staining (brown) in each tissue. The results show that human EpCAM is highly expressed in the intestine and kidney of hCD3EDG/hEpCAM knockin mice. (40x Magnification)  

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