MHCII/B2m-KO(NMG)

Nomenclature

NOD.Cg-PrkdcscidIl2rgem1H2-Ab1em1H2-Aaem1H2-Eb1em1H2-Eb2em1H2-Ea-ps em1B2m em1Smoc

Cat. NO.

NM-NSG-014

Strain State

Repository Live

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Model Description

All sequences from the upstream of mouse H2-Ab1 gene exon 2 to the upstream of the H2-Ea-ps gene exon 1 were knocked out to obtain H2-Ab1、H2-Aa、H2-Eb1、H2-Eb2、H2-Ea-ps knockout mouse models.Also, B2m gene was knocked out. These MHC class I and II molecule-deficient mice were on the NMG genetic background and also lacked cell surface expression of both class II-A and class II-E MHC proteins and B2m of class I.

Validation Data

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Fig1. Detection of MHCII and B2m expression in spleen in MHCII/B2M-dKO (NMG) mice by RT-PCR. 

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Fig2. Expression characterization of mouse MHC I and MHC II  in MHCII/B2M-dKO (NMG) mice.

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Fig3. Hematology of MHCII/B2M-dKO (NMG) mice.

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Fig4. Blood biochemistry of MHCII/B2M-dKO (M-NSG) mice.

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Fig5. Survival rate of huPBMC-MHCII/B2M-dKO (NMG) mice.

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Fig6. GvHD scores of huPBMC-MHCII/B2M-dKO (NMG) mice.

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Fig6. Body weight growth curve of huPBMC-MHCII/B2M-dKO (NMG) mice

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